Quantitative T-cell interferon-gamma responses to Mycobacterium tuberculosis-specific antigens in active and latent tuberculosis

  • C. B. E. Chee
  • T. M. S. Barkham
  • K. W. KhinMar
  • S. H. Gan
  • Y. T. Wang
Concise Article

DOI: 10.1007/s10096-008-0670-8

Cite this article as:
Chee, C.B.E., Barkham, T.M.S., KhinMar, K.W. et al. Eur J Clin Microbiol Infect Dis (2009) 28: 667. doi:10.1007/s10096-008-0670-8

Abstract

The objective was to compare the quantitative T-cell responses measured by the commercial interferon-gamma (IFNγ) release assays (IGRAs) in active and latent tuberculosis (TB) states. T-cell responses of culture-proven TB cases were compared with those of contacts with positive IGRA results and tuberculin skin tests ≥ 15 mm. T-SPOT.TB results in 270 active TB cases and 183 community contacts showed the median spot-forming cells (SFCs) above negative control/2.5 × 105 peripheral blood mononuclear cells to be 27 (−1 to 203) vs 10 (−2 to 174) in response to ESAT-6 (p < 0.001); and 37 (0 to 293) vs 13 (0 to 225) to CFP-10 (p < 0.001). The median IFNγ levels (antigen minus nil control) as measured by QuantiFERON-TB Gold In-tube in 270 cases and 142 contacts in congregate settings was 2.3 IU/ml (−0.58 to 31.44) vs 1.7 IU/ml (0.35 to 26.51, p = 0.98). Quantitative T-cell responses as measured by the T-SPOT.TB may indicate mycobacterial burden and disease activity, but cannot be used to discriminate active from latent TB.

Copyright information

© Springer-Verlag 2008

Authors and Affiliations

  • C. B. E. Chee
    • 1
  • T. M. S. Barkham
    • 2
  • K. W. KhinMar
    • 1
  • S. H. Gan
    • 1
  • Y. T. Wang
    • 1
  1. 1.Singapore Tuberculosis Control UnitTan Tock Seng HospitalSingaporeSingapore
  2. 2.Department of Laboratory MedicineTan Tock Seng HospitalSingaporeSingapore

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