The in vitro susceptibility of Campylobacter spp. to the antibacterial effect of manuka honey
- 222 Downloads
We report the antimicrobial effect of manuka honey against Campylobacter spp. isolated by a diagnostic laboratory from specimens from a community in New Zealand. The isolates were differentiated according to species level using multiplex PCR. C. jejuni (20 strains) and C. coli (7 strains) were identified. The clinical isolates identified and type culture collection strains of these species were subjected to testing to determine the minimum inhibitory concentration (MIC) of manuka honey using a microdilution technique. The MIC of the manuka honey against all of the Campylobacter tested was found to be around 1% (v/v) honey. The low MIC values suggest that honey might still inhibit the growth of campylobacteria after dilution by fluid in the gut, but the actual concentration of honey that can be achieved in the intestine is unknown. Therefore, clinical investigation is required to establish the efficacy of honey against Campylobacter spp. in the gut environment.
We thank Chris Pickett and the staff of Medlab, Hamilton, New Zealand, for advice and for the provision of the campylobacteria cultures.
- 3.Institute of Environmental Science and Research (2006) Notifiable and other diseases in New Zealand—annual report 2005. Population and Environmental Health Group, Institute of Environmental Science and Research, pp 1–56Google Scholar
- 4.Robinson DA (1981) Infective dose of Campylobacter jejuni in milk. Br Med J (Clin Res Ed) 282:1584Google Scholar
- 16.Molan PC (1992) The antibacterial activity of honey. I. The nature of the antibacterial activity. Bee World 73(1):5–28Google Scholar
- 17.Adebolu TT (2005) Effect of natural honey on local isolates of diarrhea-causing bacteria in southwestern Nigeria. Afr J Biotechnol 4(10):1172–1174Google Scholar
- 18.Molan PC (1992) The antibacterial activity of honey. II. Variation in the potency of the antibacterial activity. Bee World 73(2):59–76Google Scholar
- 22.National Committee for Clinical Laboratory Standards (2002) Performance standards for antimicrobial disk and dilution susceptibility tests for bacteria isolated from animals: approved standard (M31-A2). NCCLS, Wayne, PAGoogle Scholar
- 26.Allen KL, Molan PC, Reid GM (1991) The variability of the antibacterial activity of honey. Apiacta XXVI:114–121Google Scholar
- 30.Wang G, Clark CG, Taylor TM, Pucknell C, Barton C, Price L et al (2002) Colony multiplex PCR assay for identification and differentiation of Campylobacter jejuni, C. coli, C. lari, C. upsaliensis, and C. fetus subsp. fetus. J Clin Microbiol 40(12):4744–4747 doi:10.1128/JCM.40.12.4744-4747.2002 PubMedCrossRefGoogle Scholar
- 32.R Development Core Team (2007) R: a language and environment for statistical computing. R Foundation for Statistical Computing, ViennaGoogle Scholar
- 41.Cuccurullo SJ (2004) Physical medicine and rehabilitation board review. Demos Medical Publishing, New YorkGoogle Scholar