Quantitative variations in the vaginal bacterial population associated with asymptomatic infections: a real-time polymerase chain reaction study

  • E. Biagi
  • B. Vitali
  • C. Pugliese
  • M. Candela
  • G. G. G. Donders
  • P. Brigidi
Concise Article


The real-time polymerase chain reaction (PCR) quantification of several vaginal bacterial groups in healthy women and patients developing asymptomatic bacterial vaginosis (BV) and candidiasis (CA) was performed. Statistical analysis revealed that the BV condition is characterised by a great variability among subjects and that it is associated with a significant increase of Prevotella, Atopobium, Veillonella and Gardnerella vaginalis, and a drop in Lactobacillus. On the contrary, the vaginal microflora of healthy women and patients developing CA was found to be homogeneous and stable over time.


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Copyright information

© Springer-Verlag 2008

Authors and Affiliations

  • E. Biagi
    • 1
  • B. Vitali
    • 1
  • C. Pugliese
    • 1
  • M. Candela
    • 1
  • G. G. G. Donders
    • 2
    • 3
  • P. Brigidi
    • 1
  1. 1.Department of Pharmaceutical SciencesUniversity of BolognaBolognaItaly
  2. 2.Femicare Clinical Research for WomenTienenBelgium
  3. 3.Departments of Obstetrics and GynaecologyGeneral Hospital Heilig Hart Tienen and University Hospital Gasthuisberg, Catholic University of LeuvenLeuvenBelgium

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