Using a multiplex polymerase chain reaction for the identification of Beijing strains of Mycobacterium tuberculosis

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Abstract

The genotype of a Beijing strain of Mycobacterium tuberculosis (MTB) is usually determined by spoligotyping. However, this technique requires special equipment and is time-consuming. In this study, we developed a new multiplex polymerase chain reaction (PCR) to differentiate between Beijing and non-Beijing strains of MTB. A total of 323 MTB isolates were genotyped by both spoligotyping and the novel multiplex PCR. By spoligotyping, 169 (52.3%) isolates were determined to be Beijing strains and the remaining 154 (47.7%) isolates were non-Beijing strains. The multiplex PCR method produced results identical to those of spoligotyping in the identification of Beijing strains of MTB. This method is highly sensitive, specific, and fast. It is also cost-effective and suitable for screening large numbers of samples.

Keywords

Polymerase Chain Reaction Polymerase Chain Reaction Product Multiplex Polymerase Chain Reaction Polymerase Chain Reaction Primer Rv2819 Gene 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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Notes

Acknowledgments

This study was supported by grants DOH95-DC-1601 from the Department of Health and TSGH-C95-40 from the Tri-Service General Hospital, Taiwan. We thank Dr. Chao-Hung Lee for the assistance with the manuscript.

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Copyright information

© Springer-Verlag 2008

Authors and Affiliations

  1. 1.Division of Clinical Pathology, Department of PathologyTri-Service General HospitalTaipeiROC
  2. 2.Division of Clinical ResearchNational Health Research InstitutesZhunanROC
  3. 3.Department of Laboratory MedicineChina Medical University HospitalTaichungROC

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