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Preliminary investigations of the colonisation of upper respiratory tract tissues of infants using a paediatric formulation of the oral probiotic Streptococcus salivarius K12

  • D. A. Power
  • J. P. Burton
  • C. N. Chilcott
  • P. J. Dawes
  • J. R. Tagg
Brief Report

Abstract

A powder preparation of the oral probiotic Streptococcus salivarius K12 has been given to 19 young otitis media-prone children following a 3-day course of amoxicillin administered as a preliminary to ventilation tube placement. In two subjects, the use of strain K12 appeared to effect the expansion of an indigenous population of inhibitory S. salivarius. In other children, strain K12 colonisation extended beyond the oral cavity to also include the nasopharynx or adenoid tissue. The relatively low proportion (33%) of subjects that colonised was attributed to failure of the amoxicillin pre-treatment to sufficiently reduce the indigenous S. salivarius populations prior to dosing with strain K12 powder.

Keywords

Infective Endocarditis Eric Profile SalB Enterobacterial Repetitive Intergenic Consensus Adenoid Tissue 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgements

We gratefully acknowledge the assistance provided by nurse Rhonda Stafford in recruiting the patients.

References

  1. 1.
    Roberts JE, Rosenfeld RM, Zeisel SA (2004) Otitis media and speech and language: a meta-analysis of prospective studies. Pediatrics 113:e238–e248. DOI  10.1542/peds.113.3.e238 PubMedCrossRefGoogle Scholar
  2. 2.
    Roos K, Håkansson EG, Holm S (2001) Effect of recolonisation with “interfering” alpha streptococci on recurrences of acute and secretory otitis media in children: randomised placebo controlled trial. BMJ 322:210–212. DOI  10.1136/bmj.322.7280.210 PubMedCrossRefGoogle Scholar
  3. 3.
    Roos K, Holm SE, Grahn-Håkansson E, Lagergren L (1996) Recolonization with selected alpha-streptococci for prophylaxis of recurrent streptococcal pharyngotonsillitis—a randomized placebo-controlled multicentre study. Scand J Infect Dis 28:459–462. DOI  10.3109/00365549609037940 PubMedCrossRefGoogle Scholar
  4. 4.
    Douglas CW, Heath J, Hampton KK, Preston FE (1993) Identity of viridans streptococci isolated from cases of infective endocarditis. J Med Microbiol 39:179–182PubMedCrossRefGoogle Scholar
  5. 5.
    Burton JP, Wescombe PA, Moore CJ, Chilcott CN, Tagg JR (2006) Safety assessment of the oral cavity probiotic Streptococcus salivarius K12. Appl Environ Microbiol 72:3050–3053. DOI  10.1128/AEM.72.4.3050-3053.2006 PubMedCrossRefGoogle Scholar
  6. 6.
    Wescombe PA, Burton JP, Cadieux PA, Klesse NA, Hyink O, Heng NC, Chilcott CN, Reid G, Tagg JR (2006) Megaplasmids encode differing combinations of lantibiotics in Streptococcus salivarius. Antonie Van Leeuwenhoek 90:269–280. DOI  10.1007/s10482-006-9081-y PubMedCrossRefGoogle Scholar
  7. 7.
    Hyink O, Wescombe PA, Upton M, Ragland N, Burton JP, Tagg JR (2007) Salivaricin A2 and the novel lantibiotic salivaricin B are encoded at adjacent loci on a 190-kilobase transmissible megaplasmid in the oral probiotic strain Streptococcus salivarius K12. Appl Environ Microbiol 73:1107–1113. DOI  10.1128/AEM.02265-06 PubMedCrossRefGoogle Scholar
  8. 8.
    Tagg JR, Pybus V, Phillips LV, Fiddes TM (1983) Application of inhibitor typing in a study of the transmission and retention in the human mouth of the bacterium Streptococcus salivarius. Arch Oral Biol 28:911–915. DOI  10.1016/0003-9969(83)90086-9 PubMedCrossRefGoogle Scholar
  9. 9.
    de Bruijn FJ (1992) Use of repetitive (repetitive extragenic palindromic and enterobacterial repetitive intergeneric consensus) sequences and the polymerase chain reaction to fingerprint the genomes of Rhizobium meliloti isolates and other soil bacteria. Appl Environ Microbiol 58:2180–2187PubMedGoogle Scholar
  10. 10.
    Dierksen KP, Moore CJ, Inglis M, Wescombe PA, Tagg JR (2007) The effect of ingestion of milk supplemented with salivaricin A-producing Streptococcus salivarius on the bacteriocin-like inhibitory activity of streptococcal populations on the tongue. FEMS Microbiol Ecol 59:584–591. DOI  10.1111/j.1574-6941.2006.00228.x PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag 2008

Authors and Affiliations

  • D. A. Power
    • 1
  • J. P. Burton
    • 2
  • C. N. Chilcott
    • 2
  • P. J. Dawes
    • 3
  • J. R. Tagg
    • 1
    • 2
  1. 1.Department of Microbiology and ImmunologyUniversity of OtagoDunedinNew Zealand
  2. 2.BLIS Technologies Ltd.DunedinNew Zealand
  3. 3.Otolaryngology and Head and Neck Surgery, Department of Medical and Surgical SciencesUniversity of OtagoDunedinNew Zealand

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