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Molecular characterisation of linezolid resistance in two vancomycin-resistant (VanB) Enterococcus faecium isolates using Pyrosequencing™

  • B. Saager
  • H. Rohde
  • B. S. Timmerbeil
  • G. Franke
  • W. Pothmann
  • J. Dahlke
  • S. Scherpe
  • I. Sobottka
  • P. Heisig
  • M. A. Horstkotte
Concise Article

Abstract

In this paper, we describe the phenotypic and molecular characteristics of two clinically relevant, vancomycin-resistant (VanB), linezolid-resistant Enterococcus faecium isolates. Pyrosequencing™ showed the G to T single nucleotide polymorphism at bp 2576 in the genes coding for 23S rRNA and was used to quantify the proportion of G to T mutations among six different 23S rRNA genes in E. faecium as a marker for the molecular level of resistance to linezolid. In both isolates, the G to T mutation was found in two of six alleles, and no further mutations in the genes coding for 23S rRNA were found. The dynamic process of linezolid resistance could be demonstrated by the complete reversion of resistant alleles back to only wild type alleles in consecutive isolates of one isolate. Pyrosequencing™ being used to detect and quantify resistance to linezolid has been proven as a fast and reliable molecular screening method for monitoring linezolid resistance.

Keywords

Linezolid Teicoplanin Daptomycin Glycopeptide Resistance Linezolid Resistance 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgements

We thank Elke Halle, Institute for Microbiology and Hygiene, Charite Medical University, Berlin, Germany, for kindly providing the reference strains of linezolid-resistant enterococci.

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Copyright information

© Springer-Verlag 2008

Authors and Affiliations

  • B. Saager
    • 1
  • H. Rohde
    • 2
  • B. S. Timmerbeil
    • 1
  • G. Franke
    • 2
  • W. Pothmann
    • 3
  • J. Dahlke
    • 4
  • S. Scherpe
    • 2
  • I. Sobottka
    • 2
  • P. Heisig
    • 1
  • M. A. Horstkotte
    • 2
    • 5
  1. 1.Department of Pharmaceutical Biology and MicrobiologyUniversity of HamburgHamburgGermany
  2. 2.Department of Medical Microbiology, Virology and HygieneUniversity Medical Center Hamburg-EppendorfHamburgGermany
  3. 3.Department of Intensive Care MedicineUniversity Medical Center Hamburg-EppendorfHamburgGermany
  4. 4.Department of Bone Marrow TransplantationUniversity Medical Center Hamburg-EppendorfHamburgGermany
  5. 5.Bioscientia Institut für Medizinische Diagnostik GmbHBioscientia MVZ HamburgHamburgGermany

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