The presence of infectious extracellular Francisella tularensis subsp. novicida in murine plasma after pulmonary challenge

  • J.-J. Yu
  • E. K. Raulie
  • A. K. Murthy
  • M. N. Guentzel
  • K. E. Klose
  • B. P. ArulanandamEmail author
Brief Report

Francisella tularensis is a gram-negative, facultative intracellular bacterium and is the causative agent of the zoonotic disease tularemia [1]. F. tularensis has been considered a potential biological weapon due to its low infectious dose and high mortality rate [2]. F. tularensis can be classified into several subspecies, including those relevant to human disease: F. tularensis subsp. tularensis (type A) and F. tularensis subsp. holarctica (type B); F. novicida and F. mediasiatica [3]. However, most of our knowledge about the pathogenesis of Francisella and the immune responses to the infection have come from studies of F. tularensis LVS (derived from holartica) and F. novicida [4, 5, 6]. Both organisms are attenuated in humans, while retaining virulence in mice. It is well established that Francisellaenters and replicates within host cells, and a strong research focus has been on deciphering the mechanisms for intramacrophage growth. However, little is known about the...


Intracellular Bacterium Tularemia Francisella Tularensis Secondary Organ Tularensis Subsp 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



This work was supported by National Institutes of Health grant PO1 AI057986. The authors thank Michael Pammit from UTSA for technical expertise.


  1. 1.
    Tarnvik A (1989) Nature of protective immunity to Francisella tularensis. Rev Infect Dis 11:440–451PubMedGoogle Scholar
  2. 2.
    Dennis DT, Inglesby TV, Henderson DA et al (2001) Tularemia as a biological weapon: medical and public health management. JAMA 285:2763–2773PubMedCrossRefGoogle Scholar
  3. 3.
    Titball RW, Johansson A, Forsman M (2003) Will the enigma of Francisella tularensis virulence soon be solved? Trends Microbiol 11:118–123PubMedCrossRefGoogle Scholar
  4. 4.
    Elkins KL, Cowley SC, Bosio CM (2003) Innate and adaptive immune responses to an intracellular bacterium, Francisella tularensis live vaccine strain. Microbes Infect 5:135–142PubMedCrossRefGoogle Scholar
  5. 5.
    Lauriano CM, Barker JR, Yoon SS, Nano FE, Arulanandam BP, Hassett DJ, Klose KE (2004) MglA regulates transcription of virulence factors necessary for Francisella tularensis intraamoebae and intramacrophage survival. Proc Natl Acad Sci USA 101:4246–4249PubMedCrossRefGoogle Scholar
  6. 6.
    Pammit MA, Raulie EK, Lauriano CM, Klose KE, Arulanandam BP (2006) Intranasal vaccination with a defined attenuated Francisella novicida strain induces gamma interferon-dependent antibody-mediated protection against tularemia. Infect Immun 74:2063–2071PubMedCrossRefGoogle Scholar
  7. 7.
    Provenza MJ, Klotz SA, Penn RL (1986) Isolation of Francisella tularensis from blood. J Clin Microbiol 24:453–455PubMedGoogle Scholar
  8. 8.
    Long GW, Oprandy JJ, Narayanan RB, Fortier AH, Porter KR, Nacy CA (1993) Detection of Francisella tularensis in blood by polymerase chain reaction. J Clin Microbiol 31:152–154PubMedGoogle Scholar
  9. 9.
    Forestal CA, Malik M, Catlett SV, Savitt AG, Benach JL, Sellati TJ, Furie MB (2007) Francisella tularensis has a significant extracellular phase in infected mice. J Infect Dis 196:134–137PubMedCrossRefGoogle Scholar
  10. 10.
    Forsman M, Sandstrom G, Sjostedt A (1994) Analysis of 16S ribosomal DNA sequences of Francisella strains and utilization for determination of the phylogeny of the genus and for identification of strains by PCR. Int J Syst Bacteriol 44:38–46PubMedCrossRefGoogle Scholar
  11. 11.
    Edelson BT, Unanue ER (2001) Intracellular antibody neutralizes Listeria growth. Immunity 14:503–512PubMedCrossRefGoogle Scholar
  12. 12.
    Li JS, Winslow GM (2003) Survival, replication, and antibody susceptibility of Ehrlichia chaffeensis outside of host cells. Infect Immun 71:4229–4237PubMedCrossRefGoogle Scholar
  13. 13.
    Kirimanjeswara GS, Golden JM, Bakshi CS, Metzger DW (2007) Prophylactic and therapeutic use of antibodies for protection against respiratory infection with Francisella tularensis. J Immunol 179:532–539PubMedGoogle Scholar
  14. 14.
    Stenmark S, Lindgren H, Tarnvik A, Sjostedt A (2003) Specific antibodies contribute to the host protection against strains of Francisella tularensis subspecies holarctica. Microb Pathog 35:73–80PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  • J.-J. Yu
    • 1
  • E. K. Raulie
    • 1
  • A. K. Murthy
    • 1
  • M. N. Guentzel
    • 1
  • K. E. Klose
    • 1
  • B. P. Arulanandam
    • 1
    Email author
  1. 1.Department of Biology, South Texas Center for Emerging Infectious DiseasesUniversity of Texas at San AntonioSan AntonioUSA

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