Detection by PCR of eight groups of enteric pathogens in 4,627 faecal samples: re-examination of the English case-control Infectious Intestinal Disease Study (1993–1996)

  • C. F. L. Amar
  • C. L. East
  • J. Gray
  • M. Iturriza-Gomara
  • E. A. Maclure
  • J. McLauchlin
Article

Abstract

The English case-control Infectious Intestinal Disease Study (1993–1996) failed to detect an enteric pathogen or toxin in 49% of cases of gastroenteritis. In the present study, polymerase chain reaction (PCR) assays were applied to DNA and cDNA generated from 4,627 faecal samples from cases and controls archived during the original study for the detection of norovirus, rotavirus, sapovirus, Campylobacter spp., Salmonella spp., enteroaggregative Escherichia coli, Cryptosporidium spp., and Giardia spp. The percentage of archived samples from cases and from controls in which at least one agent (or toxin) was detected increased from 53% in the original study to 75% and from 19 to 42%, respectively, after the application of PCR assays. Among cases, the following percentages of enteric pathogens were detected: norovirus 36%, rotavirus A 31%, sapovirus 4%, Salmonella spp. 6%, Campylobacter jejuni 13%, Campylobacter coli 2%, other Campylobacter spp. 8%, enteroaggregative E. coli 6%, Giardia spp. 2%, and Cryptosporidium spp. 2%. The present study provides additional insight into the aetiology of infectious intestinal disease in England and highlights the occurrence of viral infections in cases as well as in asymptomatic individuals. Other notable findings include the frequent presence of Campylobacter spp. other than C. jejuni or C. coli, the high frequency of multiple agents in 41% of cases and in 13% of controls, and the variation in the aetiology and rate of infection found for different age groups. The results demonstrate the greater sensitivity of PCR-based methods compared to current conventional methods.

Keywords

Polymerase Chain Reaction Faecal Sample Gastroenteritis Polymerase Chain Reaction Assay Enteric Pathogen 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgement

This project was funded by the Food Standards Agency, UK (project codes B14004 and B14005).

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Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  • C. F. L. Amar
    • 1
  • C. L. East
    • 1
  • J. Gray
    • 1
  • M. Iturriza-Gomara
    • 1
  • E. A. Maclure
    • 1
  • J. McLauchlin
    • 1
  1. 1.Health Protection Agency Centre for InfectionsLondonUK

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