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“All-in-one-plate” E-test and disk diffusion susceptibility co-testing for multiresistant Acinetobacter baumannii

  • J. GiladEmail author
  • M. Giladi
  • F. Poch
  • Y. Aharoni
  • D. Schwartz
Concise Article

Abstract

Multiresistant Acinetobacter baumannii requires ancillary manual susceptibility testing with the E-test and disk diffusion when tested with the VITEK-2 system (bioMérieux, Marcy l’Etoile, France). In the study presented here, the E-test and disk diffusion were combined in a single plate, and the method was verified by comparing categorical agreement of combined and control plates. There were no very major, major or minor errors, and similar results were obtained for all ten representative bacterial strains used as controls. Co-testing is thus feasible, accurate and reproducible, and it merits evaluation with other bacterial species.

Keywords

Minocycline Meropenem Colistin Disk Diffusion Tigecycline 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1. 1.
    Bergogne-Berezin E, Towner KJ (1996) Acinetobacter spp. as nosocomial pathogens: microbiological, clinical, and epidemiological features. Clin Microbiol Rev 9:148–165PubMedGoogle Scholar
  2. 2.
    Abbo A, Navon-Venezia S, Hammer-Muntz O, Krichali T, Siegman-Igra Y, Carmeli Y (2005) Multidrug-resistant Acinetobacter baumannii. Emerg Infect Dis 11:22–29PubMedGoogle Scholar
  3. 3.
    Steward CD, Mohammed JM, Swenson JM, Stocker SA, Williams PP, Gaynes RP, McGowan Jr JE, Tenover FC (2003) Antimicrobial susceptibility testing of carbapenems: multicenter validity testing and accuracy levels of five antimicrobial test methods for detecting resistance in Enterobacteriaceae and Pseudomonas aeruginosa isolates. J Clin Microbiol 41:351–358PubMedCrossRefGoogle Scholar
  4. 4.
    Clinical Laboratory Standards Institute (2006) Performance standards for antimicrobial susceptibility testing. Sixteenth informational supplement M100-S16. CLSI, Wayne, PAGoogle Scholar
  5. 5.
    AB Biodisk (2006) E-test technical manual. AB Biodisk, Solna, SwedenGoogle Scholar
  6. 6.
    Andrews JM, the BSAC Working Party on Susceptibility Testing (2005) BSAC standardized disc susceptibility testing method. J Antimicrob Chemother 56:60–76PubMedCrossRefGoogle Scholar
  7. 7.
    Clinical Laboratory Standards Institute (2006) Performance standards for antimicrobial disk susceptibility tests. Approved standard M2-A9. CLSI, Wayne, PAGoogle Scholar
  8. 8.
    Jorgensen JH (1993) Selection criteria for an antimicrobial susceptibility testing system. J Clin Microbiol 31:2841–2844PubMedGoogle Scholar
  9. 9.
    Food and Drug Administration (2003) Class II special controls guidance document: antimicrobial susceptibility test (AST) systems; guidance for industry and FDA. FDA, Rockville, MDGoogle Scholar
  10. 10.
    Van Looveren M, Goossens H, the ARPAC Steering Group (2004) Antimicrobial resistance of Acinetobacter spp. in Europe. Clin Microbiol Infect 10:684–704PubMedCrossRefGoogle Scholar
  11. 11.
    Tsakris A, Pantazi A, Pournaras S, Maniatis A, Polyzou A, Sofianou D (2000) Pseudo-outbreak of imipenem-resistant Acinetobacter baumannii resulting from false susceptibility testing by a rapid automated system. J Clin Microbiol 38:3505–3507PubMedGoogle Scholar
  12. 12.
    Gales AC, Reis AO, Jones RN (2001) Contemporary assessment of antimicrobial susceptibility testing methods for polymyxin B and colistin: review of available interpretative criteria and quality control guidelines. J Clin Microbiol 39:183–190PubMedCrossRefGoogle Scholar
  13. 13.
    Higgins PG, Wisplinghoff H, Stefanik D, Seifert H (2004) In vitro activities of the beta-lactamase inhibitors clavulanic acid, sulbactam, and tazobactam alone or in combination with beta-lactams against epidemiologically characterized multidrug-resistant Acinetobacter baumannii strains. Antimicrob Agents Chemother 48:1586–1592PubMedCrossRefGoogle Scholar
  14. 14.
    Arroyo LA, Garcia-Curiel A, Pachon-Ibanez ME, Llanos C, Ruiz M, Pachon J, Aznar J (2005) Reliability of the E-test method for detection of colistin resistance in clinical isolates of Acinetobacter baumannii. J Clin Microbiol 43:903–905PubMedCrossRefGoogle Scholar
  15. 15.
    Jones RN, for the College of American Pathologists Microbiology Resource Committee (2001) Method preferences and test accuracy of antimicrobial susceptibility testing. Arch Pathol Lab Med 125:1285–1289PubMedGoogle Scholar

Copyright information

© Springer-Verlag 2006

Authors and Affiliations

  • J. Gilad
    • 1
    Email author
  • M. Giladi
    • 1
    • 2
  • F. Poch
    • 1
  • Y. Aharoni
    • 1
  • D. Schwartz
    • 1
  1. 1.Microbiology LaboratoryTel-Aviv Sourasky Medical CenterTel-AvivIsrael
  2. 2.Infectious Disease UnitTel-Aviv Sourasky Medical CenterTel-AvivIsrael

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