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Diagnosis of pulmonary infection with Toxoplasma gondii in immunocompromised HIV-positive patients by real-time PCR

  • E. Petersen
  • B. Edvinsson
  • B. Lundgren
  • T. Benfield
  • B. EvengårdEmail author
Concise Article

Abstract

The aim of the study presented here was to evaluate the use of PCR for improving the diagnosis of Toxoplasma gondii infection in immunocompromised hosts. Three hundred thirty-two bronchoalveolar lavage (BAL) fluid samples were analyzed by real-time PCR targeting a 529 bp element of T. gondii. In positive samples, the genotype of the parasite was determined by sequence analysis of the GRA6 gene. Positive results were achieved for 2% (7/332) of the samples tested. Genotyping was possible in two samples and revealed GRA6 type II T. gondii. PCR for detecting T. gondii in BAL samples should be performed in all immunosuppressed HIV-positive patients with symptoms of a systemic infection of unknown etiology. Trimethoprim-sulfamethoxazole prophylaxis does not exclude concomitant infection with T. gondii.

Keywords

Toxoplasmosis Pyrimethamine Genome Equivalent Internal Amplification Control Pneumocystis Jirovecii 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgements

The authors are grateful to the Copenhagen University Hospital, Hvidovre HIV cohort study group for providing access to the samples.

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Copyright information

© Springer-Verlag 2006

Authors and Affiliations

  • E. Petersen
    • 1
    • 2
  • B. Edvinsson
    • 3
  • B. Lundgren
    • 5
  • T. Benfield
    • 4
  • B. Evengård
    • 3
    Email author
  1. 1.Department of Clinical Bacteriology F82Karolinska Institutet at Karolinska University Hospital—HuddingeStockholmSweden
  2. 2.Department of Infectious DiseasesAarhus University Hospital—SkejbyAarhus NDenmark
  3. 3.Department of Laboratory Medicine F82Karolinska Institute at Karolinska University Hospital—HuddingeStockholmSweden
  4. 4.Department of Infectious Diseases and Copenhagen HIV ProgrammeHvidovre University Hospital—HvidovreHvidovreDenmark
  5. 5.Department of Clinical MicrobiologyHvidovre University Hospital—HvidovreHvidovreDenmark

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