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Evaluation of a new chromogenic medium for isolation and identification of common urinary tract pathogens

  • P. CiragilEmail author
  • M. Gul
  • M. Aral
  • H. Ekerbicer
Concise Article

Abstract

In the study presented here, a new chromogenic medium (CPS ID 3; bioMérieux, Marcy l’Etoile, France) was compared to routine media for the isolation, identification and antimicrobial susceptibility testing of bacteria recovered from urine specimens, and a cost analysis was performed. Escherichia coli, Proteeae tribe, Pseudomonas aeruginosa, Enterococcus spp. and Streptococcus agalactiae grew on the chromogenic medium as typical differentiated colonies and were accurately identified even in mixed cultures. Although the similarity of colors produced by isolates belonging to the Klebsiella, Enterobacter, Serratia and Citrobacter (KESC) group prevents differentiation among them, members of KESC were easily identified as coliforms. No substantial difference was observed when comparing the results of antimicrobial susceptibility testing performed on colonies selected from reference media versus CPS ID 3. Use of the new medium was associated with a savings of 75% over the conventional methods and the API system. Furthermore, this medium facilitated a remarkable reduction in the laboratory workload and consequently resulted in additional time and cost savings.

Keywords

Mixed Culture Susceptibility Testing Urine Specimen Microbiology Laboratory Reference Medium 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgements

We would like to acknowledge the material support provided by bioMérieux for this project and thank H. Bilgehan in particular for his assistance in this regard.

References

  1. 1.
    Emori TG, Gaynes RP (1993) An overview of nosocomial infections, including the role of the microbiology laboratory. Clin Microbiol Rev 6:428–442PubMedGoogle Scholar
  2. 2.
    Scarparo C, Piccoli P, Ricordi P, Scagnelli M (2002) Comparative evaluation of two commercial chromogenic media for detection and presumptive identification of urinary tract pathogens. Eur J Clin Microbiol Infect Dis 21:283–289CrossRefPubMedGoogle Scholar
  3. 3.
    Chaux C, Crepy M, Xueref S, Roure C, Gille Y, Freydiere AM (2002) Comparison of three chromogenic agar plates for isolation and identification of urinary tract pathogens. Clin Microbiol Infect 8:641–645CrossRefPubMedGoogle Scholar
  4. 4.
    Reisner SB, Austin EF (1997) Evaluation of CPS ID2 chromogenic agar as a single medium for urine culture. Diagn Microbiol Infect Dis 28:113–117CrossRefPubMedGoogle Scholar
  5. 5.
    Perry JD, Butterworth LA, Nicholson A, Appleby MR, Orr KE (2003) Evaluation of a new chromogenic medium, Uriselect 4, for the isolation and identification of urinary tract pathogens. J Clin Pathol 56:528–531CrossRefPubMedGoogle Scholar
  6. 6.
    Fallon D, Ackland G, Andrews N, Frodsham D, Howe S, Howells K, Nye KJ, Warren RE (2003) A comparison of performance of commercially available chromogenic agars for the isolation and presumptive identification of organisms from urine. J Clin Pathol 56:608–612CrossRefPubMedGoogle Scholar
  7. 7.
    Aspevall O, Osterman B, Dittmer R, Stén L, Lindback E, Forsum U (2002) Performance of four chromogenic urine culture media after one or two days of incubation compared with reference media. J Clin Microbiol 40:1500–1503CrossRefPubMedGoogle Scholar
  8. 8.
    Warren JW (2000) Nosocomial urinary tract infections. In: Mandell GL, Bennett JE, Dolin R (eds) Principles and practice of infectious diseases, vol 2. 5th edn. Churchill Livingstone, Philadelphia, Pennsylvania, USA, pp 3028–3038Google Scholar

Copyright information

© Springer-Verlag 2006

Authors and Affiliations

  1. 1.Department of Clinical MicrobiologyKahramanmaras Sutcu Imam University, Faculty of MedicineKahramanmarasTurkey
  2. 2.Department of Public HealthKahramanmaras Sutcu Imam University, Faculty of MedicineKahramanmarasTurkey

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