Molecular detection of Bartonella quintana DNA in the dental pulp of a homeless patient

  • G. Aboudharam
  • P.-E. Fournier
  • M. Drancourt
  • D. Raoult
  • C. Foucault
  • P. Brouqui
Concise Article

Abstract

Dental pulp has been proposed as a suitable tissue sample for the identification of pathogenic organisms. Using PCR with two specific gene targets, Bartonella quintana DNA was detected in the dental pulp extracted from the tooth of a homeless patient. The patient had been bacteremic 6 months previously but was not when the tooth was sampled.

References

  1. 1.
    Anderson BE, Neuman MA (1997) Bartonella spp. as emerging human pathogens. Clin Microbiol Rev 10:203–219PubMedGoogle Scholar
  2. 2.
    Maurin M, Raoult D (1996) Bartonella (Rochalimaea) quintana infections. Clin Microbiol Rev 9:273–292PubMedGoogle Scholar
  3. 3.
    Spach DH, Koehler JE (1998) Bartonella-associated infections. Emerg Infect Dis 12:137–155Google Scholar
  4. 4.
    Brouqui P, La Scola B, Roux V, Raoult D (1999) Chronic Bartonella quintana bacteremia in homeless patients. N Engl J Med 340:184–189CrossRefPubMedGoogle Scholar
  5. 5.
    Spach DH, Kanter AS, Dougherty MJ, Larson AM, Coyle MB, Brenner DJ, Swaminathan B, Matar GM, Welch DF, Root RK (1995) Bartonella (Rochalimaea) quintana bacteremia in inner-city patients with chronic alcoholism. N Engl J Med 332:424–428CrossRefPubMedGoogle Scholar
  6. 6.
    Drancourt M, Mainardi JL, Brouqui P, Vandenesch F, Carta A, Lehnert F, Etienne J, Goldstein F, Acar J, Raoult D (1995) Bartonella (Rochalimaea) quintana endocarditis in three homeless men. N Engl J Med 332:419–423CrossRefPubMedGoogle Scholar
  7. 7.
    Rolain JM, Foucault C, Guieu R, La Scola B, Brouqui P, Raoult D (2002) Bartonella quintana in human erythrocytes. Lancet 360:226–228CrossRefPubMedGoogle Scholar
  8. 8.
    Rolain JM, Foucault C, Brouqui P, Raoult D (2003) Erythroblast cells as a target for Bartonella quintana in homeless people. Ann N Y Acad Sci 990:485–487PubMedGoogle Scholar
  9. 9.
    Aboudharam G, La Scola B, Raoult D, Drancourt M (2000) Detection of Coxiella burnetii DNA in dental pulp during experimental bacteremia. Microb Pathog 28:249–254CrossRefPubMedGoogle Scholar
  10. 10.
    Glick M, Trope M, Pliskin ME (1989) Detection of HIV in the dental pulp of a patient with AIDS. J Am Dent Assoc 119:649–650PubMedGoogle Scholar
  11. 11.
    Drancourt M, Aboudharam G, Signoli M, Dutour O, Raoult D (1998) Detection of 400-year-old Yersinia pestis DNA in human dental pulp: an approach to the diagnosis of ancient septicemia. Proc Natl Acad Sci USA 95:12637–12640CrossRefPubMedGoogle Scholar
  12. 12.
    Maurin M, Rolain JM, Raoult D (2002) Comparison of in-house and commercial slides for detection by immunofluorescence of immunoglobulins G and M against Bartonella henselae and Bartonella quintana. Clin Diagn Lab Immunol 9:1004–1009CrossRefPubMedGoogle Scholar
  13. 13.
    Foucault C, Raoult D, Brouqui P (2003) Randomized open trial of gentamicin and doxycycline for eradication of Bartonella quintana from blood in patients with chronic bacteremia. Antimicrob Agents Chemother 47:2204–2207CrossRefPubMedGoogle Scholar
  14. 14.
    Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory Press, New YorkGoogle Scholar
  15. 15.
    Zeaiter Z, Fournier PE, Ogata H, Raoult D (2002) Phylogenetic classification of Bartonella species by comparing groEL sequences. Int J Syst Evol Microbiol 52:165–171PubMedGoogle Scholar
  16. 16.
    Minnick MF, Sappington KN, Smitherman LS, Andersson SG, Karlberg O, Carroll JA (2003) Five-member gene family of Bartonella quintana. Infect Immun 71:814–821CrossRefPubMedGoogle Scholar
  17. 17.
    Aboudharam G, Drancourt M, Raoult D (2004) Culture of C. burnetii from the dental pulp of experimentally infected guinea pigs. Microb Pathog 36:349–350CrossRefPubMedGoogle Scholar
  18. 18.
    Foucault C, Barrau K, Brouqui P, Raoult D (2002) Bartonella quintana bacteremia among homeless people. Clin Infect Dis 35:684–689CrossRefPubMedGoogle Scholar

Copyright information

© Springer-Verlag 2004

Authors and Affiliations

  • G. Aboudharam
    • 1
  • P.-E. Fournier
    • 1
  • M. Drancourt
    • 1
  • D. Raoult
    • 1
  • C. Foucault
    • 2
  • P. Brouqui
    • 2
  1. 1.Unité des Rickettsies, CNRS UMR 6020 IFR 48, Faculté de MédecineUniversité de la MéditerranéeMarseille Cedex 5France
  2. 2.Service des Maladies Infectieuses et TropicalesHôpital CHU NordMarseilleFrance

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