Cytomegalovirus Infection Induces Production of Human Interleukin-10 in Macrophages
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Earlier findings have suggested that the balance between interleukin-10 and tumor necrosis factor α levels in serum may influence the outcome of cytomegalovirus infection in renal transplant recipients. Therefore, the aim of the present study was to investigate whether human cytomegalovirus induces interleukin-10 production in macrophages. Experiments using human cytomegalovirus (strain 2006), ultraviolet-inactivated cytomegalovirus, and mock-infected differentiated THP-1 cells with or without ganciclovir or monoclonal anti-tumor necrosis factor α antibodies were performed. Cytomegalovirus-infected cells produced significantly higher levels of human interleukin-10 mRNA and interleukin-10 than ultraviolet-inactivated cytomegalovirus or mock-infected cells. The addition of ganciclovir had little effect on interleukin-10 production. Anti-tumor necrosis factor α antibodies appeared to reduce the interleukin-10 levels. In conclusion, human cytomegalovirus infection of macrophages induces production of human interleukin-10. This requires viral entry, but not full viral replication.
KeywordsGanciclovir Renal Transplant Recipient Human Cytomegalovirus Human Monocytic Leukemic Cell Line Human Embryonic Fibroblast Cell
We thank Bodil Lunden and Solveig Beck for excellent technical assistance. The present experiments comply with current Norwegian laws.
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