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Rapid Detection of Pathogenic Fungi from Clinical Specimens Using LightCycler Real-Time Fluorescence PCR

  • A. Imhof
  • C. Schaer
  • G. Schoedon
  • D. J. Schaer
  • R. B. Walter
  • A. Schaffner
  • M. SchneemannEmail author
Concise Article

Abstract

In the study presented here a LightCycler real-time PCR system was used for the diagnosis of fungal infections from clinical tissue samples. Nine specimens were investigated from six patients with suspected or proven invasive fungal infections. Seven of nine samples were positive in a broad-range fungal PCR assay. In four samples, Aspergillus fumigatus was detected both by a species-specific hybridization assay as well as by sequencing of amplification products. In addition, the broad-range fungal PCR assay and PCR sequencing detected and identified, respectively, the following organisms in the specimens noted: Candida albicans in a culture-negative liver biopsy, Histoplasma capsulatum in a bone marrow sample, and Conidiobolus coronatus in a facial soft tissue specimen. Real-time PCR is a promising tool for the diagnosis of invasive fungal infections in human tissue samples and offers some advantages over culture methods, such as rapid analysis and increased sensitivity.

Keywords

Aspergillus Fumigatus Invasive Fungal Infection Human Tissue Sample Histoplasma Capsulatum Facial Soft Tissue 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag 2003

Authors and Affiliations

  • A. Imhof
    • 1
  • C. Schaer
    • 3
  • G. Schoedon
    • 3
  • D. J. Schaer
    • 4
  • R. B. Walter
    • 2
  • A. Schaffner
    • 3
  • M. Schneemann
    • 3
    Email author
  1. 1.Program in Infectious Disease, Clinical Research DivisionFred Hutchinson Cancer Research CenterSeattleUSA
  2. 2.Pediatric Oncology, Clinical Research DivisionFred Hutchinson Cancer Research CenterSeattleUSA
  3. 3.Department of Medicine, Clinical Mycology, Medical Clinic BUniversity Hospital ZurichZurichSwitzerland
  4. 4.Department of AnesthesiologyUniversity Hospital ZurichZurichSwitzerland

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