Journal of Wood Science

, Volume 62, Issue 5, pp 460–471 | Cite as

Zn2+, a key factor of colony morphogenesis of Tricholoma matsutake

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Abstract

Tricholoma matsutake is a basidiomycete producing edible fruiting bodies known as matsutake in Japanese. Matsutake is one of the most valuable edible mushrooms in Japan. Colony morphology on agar plates of T. matsutake is known to vary depending on compositions of media used, while the mechanism is still unknown. In our present work, we have found Zn2+ affects colony morphology of T. matsutake. When Zn2+ was added at concentration more than 1 µM, colony got wrinkled and aerial hyphae formation was activated. No such morphological changes have been confirmed without Zn2+ addition or at concentration less than 1 µM, even when other nutritional ingredients are provided sufficiently. None of the Zn2+ concentrations examined present has affected colony diameter, dry weight, and water content. These findings imply Zn2+ is a key factor involved in colony morphogenesis of T. matsutake such as colony wrinkling and aerial hyphae formation. Metabolomic information obtained with gas chromatography–mass spectrometry has indicated that Zn2+ deficiency has influences on carbohydrate and l-serine metabolisms.

Keywords

Tricholoma matsutake Colony morphology Zinc Aerial hyphae Metabolomics 
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Notes

Acknowledgments

GC/MS data were acquired with equipment in the Laboratory of Systematic Forest and Forest Products Sciences in the Graduate School of Kyushu University. We’d appreciate kindness of Dr. Shimizu, an associate professor of the laboratory.

Compliance with ethical standards

Conflict of interest

The authors declare no conflicts of interest. All the experiments undertaken in this study comply with the current laws of the country where they were performed.

Funding

This work was supported by JSPS Grant-in-Aid for Challenging Exploratory Research Number 24658155.

Supplementary material

10086_2016_1571_MOESM1_ESM.eps (3.6 mb)
Supplementary material 1 (EPS 3730 kb)

References

  1. 1.
    Arai Y, Takao M, Sakamoto R, Yoshikawa K, Terashita T (2003) Promotive effect of the hot water-soluble fraction from corn fiber on vegetative mycelial growth in edible mushrooms. J Wood Sci 49:437–443CrossRefGoogle Scholar
  2. 2.
    Guerin-Laguette A, Vaario LM, Matsushita N, Shindo K, Suzuki K, Lapeyrie F (2003) Growth stimulation of a Shiro-like, mycorrhiza forming, mycelium of Tricholoma matsutake on solid substrates by non-ionic surfactants or vegetable oil. Mycol Prog 2(1):37–44CrossRefGoogle Scholar
  3. 3.
    Kawagishi H, Hamajima K, Takanami R, Nakamura T, Sato Y, Akiyama Y, Sano M, Tanaka O (2004) Growth promotion of Mycelia of Matsutake Mushroom Tricholoma matsutake by d-isoleucine. Biosci Biotechnol Biochem 68(11):2405–2407CrossRefPubMedGoogle Scholar
  4. 4.
    Vaario LM, Guerin-Laguette A, Matsushita N, Suzuki K, Lapeyrie F (2002) Saprobic potential of Tricholoma matsutake: growth over pine bark treated with surfactants. Mycorrhiza 12:1–5CrossRefPubMedGoogle Scholar
  5. 5.
    Kusuda M, Ueda M, Miyatake K, Terashita T (2008) Characterization of the carbohydrase productions of an ectomycorrhizal fungus, Tricholoma matsutake. Mycoscience 49:291–297CrossRefGoogle Scholar
  6. 6.
    Ogawa M (1978) Matsutake no seibutsugaku (in Japanese). Tsukiji Shokan, JapanGoogle Scholar
  7. 7.
    Geoffrey MG, Lynn R, John WG, Karl R (2001) Nutritional influence on fungal colony growth and biomass distribution in response to toxic metals. FEMS Microbiol Lett 204:311–316CrossRefGoogle Scholar
  8. 8.
    Ohta A (1990) A new medium for mycelial growth of mycorrhizal fungi. Trans Mycol Soc Jpn 31:323–334Google Scholar
  9. 9.
    Murashige T, Skoog F (1962) A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol Plant 15(3):473–497CrossRefGoogle Scholar
  10. 10.
    Marx DH (1969) The influence of ectotrophic mycorrhizal fungi on the resistance of pathogenic infections. I. Antagonism of mycorrhizal fungi to pathogenic fungi and soil bacteria. Phytopathology 59:153–163Google Scholar
  11. 11.
    Talbot NJ (1997) Fungal biology: growing into the air. Curr Biol 7(2):78–81CrossRefGoogle Scholar
  12. 12.
    Wessels JGH, De Vries OMH, Ásgeirsdóttir SA, Springer J (1991) The thn mutation of Schizophyllum commune, which suppresses formation of aerial hyphae, affects expression of the Sc3 hydrophobin gene. J Gen Microbiol 137:2439–2445CrossRefPubMedGoogle Scholar
  13. 13.
    Vatsala TM, Shanmugasundaram KR, Shanmugasundaram ERB (1976) Role of nicotinic acid in pyridoxine biosynthesis in Aspergillus nidulans. Biochem Biophys Res Commun 72(4):1570–1575CrossRefPubMedGoogle Scholar
  14. 14.
    Lanfranco L, Balsamo R, Martino E, Perotto S, Bonfante P (2002) Zinc ions alter morphology and chitin deposition in an ericoid fungus. Eur J Histochem 46:341–350CrossRefPubMedGoogle Scholar

Copyright information

© The Japan Wood Research Society 2016

Authors and Affiliations

  1. 1.Laboratory of Silviculture, Department of Agro-environmental Sciences, Graduate School of Bioresource and Bioenvironmental SciencesKyushu UniversityFukuokaJapan

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