Journal of Wood Science

, Volume 62, Issue 3, pp 285–293 | Cite as

Screening for melanogenesis-controlled agents using Sudanese medicinal plants and identification of active compounds in the methanol extract of Terminalia brownii bark

  • Kosei Yamauchi
  • Tohru Mitsunaga
  • Ali Mahmoud Muddathir
Note

Abstract

We isolated and identified compounds in medicinal plant extracts that could control melanogenesis. Sudanese medicinal plants were extracted with methanol (MeOH) and 50 % ethanol (EtOH)/water, yielding 104 extracts that were screened for melanogenic activity using B16 melanoma cells. The MeOH extract of Terminalia brownii bark dose-dependently enhanced intracellular and extracellular melanogenesis, with no cytotoxicity. Furthermore, we isolated and identified the components in T. brownii MeOH extract. Gallic acid (1), α,β-punicalagin (2), α,β-terchebulin (3), ellagic acid 4-O-α-l-rhamnopyranoside (4), ellagic acid (5), and 3,4,3′-tri-O-methylellagic acid (6) were isolated by chromatography and identified using nuclear magnetic resonance (NMR), matrix-assisted laser desorption/ionization (MALDI) or ultra-performance liquid chromatography–time-of-flight mass spectrometry (UPLC–TOFMS), and ultraviolet (UV) spectroscopy data. Among the isolated compounds, 2, 3, 5, and 6 enhanced melanogenesis. Furthermore, compound 1 inhibited intracellular and extracellular melanogenesis with no cytotoxicity.

Keywords

Sudanese medicinal plants B16 melanoma cell Terminalia brownii Tyrosinase 

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Copyright information

© The Japan Wood Research Society 2016

Authors and Affiliations

  • Kosei Yamauchi
    • 1
  • Tohru Mitsunaga
    • 1
  • Ali Mahmoud Muddathir
    • 2
  1. 1.The United Graduate School of Agricultural Science, Gifu UniversityGifuJapan
  2. 2.Department of Horticulture, Faculty of AgricultureUniversity of KhartoumKhartoum North-ShambatSudan

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