Journal of Wood Science

, Volume 61, Issue 2, pp 204–210 | Cite as

Hydrogen cyanide for treating wood against pine wood nematode (Bursaphelenchus xylophilus): results of a model study

  • Ondřej Douda
  • Miloslav Zouhar
  • Marie Maňasová
  • Milan Dlouhý
  • Jana Lišková
  • Pavel Ryšánek


The pine wood nematode (Bursaphelenchus xylophilus) belongs to economically most important quarantine plant parasitic nematodes in Asia and Europe. As wood transported within the international trade plays major role in B. xylophilus spreading into new areas, attention should be given to wood treatment. With methyl bromide ban in the EU in 2000 new chemicals should be investigated. This work describes results obtained from model fumigation of hollow wooden blocks containing B. xylophilus nematodes in sawdust with gaseous hydrogen cyanide (HCN). Data considering HCN concentration in gas chamber and treated wooden blocks are also presented; HCN concentrations inside wooden blocks were recounted to ct product values which show irregular sorption of HCN by the wood. Total B. xylophilus mortality was observed in the variants treated with the initial HCN concentration of 12.30 g m−3 and exposure times from 8 to 20 h, 18.21 g m−3 and exposure times of 2, 4, 6, 10 and 16 to 20 h, 21.71 g m−3 and exposure times of 12, 18 and 20 h and 24.12 g m−3 and exposure times of 2, 6, 12 and 18 h. Results show overall good efficiency of HCN treatment on B. xylophilus mortality, however, research on naturally infested wood should be desirable.


Bursaphelenchus xylophilus Methyl bromide alternative Hydrogen cyanide Fumigation Wood treatment 



This research was supported by the research project nr. QI111B065 of the Ministry of Agriculture of the Czech Republic. Authors would like to thank Mr. Pedro Barbosa and Dr. Václav Čermák for providing nematodes for the establishing of the laboratory cultures, to Ms. J. Wenzlová and Ms. Jana Vavřičková for technical assistance and to Dr. Václav Stejskal and Dr. Radek Aulický for review of the manuscript.


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Copyright information

© The Japan Wood Research Society 2015

Authors and Affiliations

  • Ondřej Douda
    • 1
  • Miloslav Zouhar
    • 2
  • Marie Maňasová
    • 2
  • Milan Dlouhý
    • 3
  • Jana Lišková
    • 3
  • Pavel Ryšánek
    • 2
  1. 1.Division of Plant HealthCrop Research Institute PraguePrague 6, RuzyněCzech Republic
  2. 2.Department of Plant Protection, Faculty of Agrobiology Food and Natural ResourcesCzech University of Life Sciences PraguePrague 6, SuchdolCzech Republic
  3. 3.Lučební závody Draslovka a.s.Kolín IVCzech Republic

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