Journal of Wood Science

, Volume 60, Issue 2, pp 169–174 | Cite as

Intsia palembanica wood extracts and its isolated compounds as Propionibacterium acnes lipase inhibitor

  • Irmanida Batubara
  • Harlinda Kuspradini
  • Ali Mahmoud Muddathir
  • Tohru Mitsunaga


Potential compounds from Intsia palembanica methanol extracts were isolated. Three isolated compounds as well as 7 reported compounds from Merbau were analyzed for their ability to inhibit the lipase activity of Propionibacterium acnes. Lipase was isolated from P. acnes and used as enzyme for activity analysis. The lipase activity test was performed using the 2,3-dimercapto-1-propanol tributyrate (BALB) method. The results showed that methanol extract and water fraction were active to inhibit lipase activity but n-hexane fraction and EtOAc fraction did not reach 50 % inhibition until 500 μg/ml. EtOAc fraction consisted of flavonoid, naringenin, robinetin, and (+)-epirobidanol accelerated the lipase activity. The other 7 compounds showed inhibitory activity of lipase in a concentration-dependent manner. In conclusion, fustin is the most active compound to inhibit the P. acnes lipase activity.


Intsia palembanica Propionibacterium acnes lipase inhibitor Flavonoid 


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Copyright information

© The Japan Wood Research Society 2014

Authors and Affiliations

  • Irmanida Batubara
    • 1
  • Harlinda Kuspradini
    • 2
  • Ali Mahmoud Muddathir
    • 3
    • 5
  • Tohru Mitsunaga
    • 4
  1. 1.Department of Chemistry, Faculty of Mathematics and Natural Sciences, Biopharmaca Research CenterBogor Agricultural UniversityBogorIndonesia
  2. 2.Faculty of ForestryMulawarman UniversitySamarindaIndonesia
  3. 3.Graduated School of Agricultural ScienceGifu UniversityGifuJapan
  4. 4.Faculty of Applied Biological ScienceGifu UniversityGifuJapan
  5. 5.Department of Horticulture, Faculty of AgricultureUniversity of KhartoumKhartoum North, ShambatSudan

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