Journal of Wood Science

, Volume 59, Issue 5, pp 426–431 | Cite as

Anti-acne activity of tannin-related compounds isolated from Terminalia laxiflora

  • Ali Mahmoud Muddathir
  • Kosei Yamauchi
  • Tohru Mitsunaga
Original article


In our investigation to find out new anti-acne agent, we focused on Terminalia laxiflora Engl & Diels (Combretaceae) methanolic wood extract, which has been selected during previous screening experiments for anti-acne agents, which included 29 species of Sudanese medicinal plants. Based on the biologically guided fractionation using an antibacterial assay against Propionibacterium acnes, a lipase inhibitory assay and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity assay; five tannin-related compounds were isolated, such as ellagic acid, flavogallonic acid dilactone, terchebulin and gallic acid. Terchebulin showed good antibacterial activity; minimum inhibitory concentration (MIC) = 125 μg/ml and minimum bactericidal concentration (MBC) = 250 μg/ml. Gallic acid exhibited lipase inhibitory activity with IC50 value of 149.3 μM, which showed strong inhibition compared with terchebulin, IC50 260.7 μM. However, all compounds exhibited better or equal DPPH radical scavenging activity to (+)-catechin as positive control. Ellagic acid and terchebulin showed the best DPPH radical scavenging activities, IC50 4.86 and 4.90 μM, respectively. This study demonstrated that terchebulin has potentiality as an anti-acne agent.


Terminalia laxiflora Engl & Diels Propionibacterium acnes Lipase inhibitor Terchebulin Flavogallonic acid dilactone 


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Copyright information

© The Japan Wood Research Society 2013

Authors and Affiliations

  • Ali Mahmoud Muddathir
    • 1
  • Kosei Yamauchi
    • 1
  • Tohru Mitsunaga
    • 2
  1. 1.United Graduate School of Agricultural Science, Gifu UniversityGifuJapan
  2. 2.Department of Applied Biological Science, Faculty of Applied Biological ScienceGifu UniversityGifuJapan

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