Journal of Wood Science

, Volume 53, Issue 3, pp 264–267 | Cite as

Transmission of mitochondrial plasmids in protoplast cell fusion between compatible monokaryons of Lentinula edodes

  • Masaki FukudaEmail author
  • Izumi Sendo
  • Satoko Imahori
  • Yukitaka Fukumasa-Nakai


Electrophoretic analysis of the transmission pattern of mitochondrial plasmids in protoplast cell fusion between compatible monokaryons of Lentinula edodes indicates that three of the four plasmids carried in parental monokaryons are effectively transferred and replicated in the protoplast fusants. The two monokaryons, 1158a and 1569a, carried different plasmids that could be distinguished by a single restriction digest. Electrophoresis of intact plasmids and restriction analyses indicate that all but one of the fusants carry three of the four possible plasmids, indicating that transmission of plasmids in protoplast fusions is principally biparental in L. edodes. Thus, heterocytoplasmic cells of L. edodes can be effectively constructed by protoplast cell fusion. In addition, plasmids of the same homology group cannot coexist in the heteroplasmic cells after protoplast cell fusion.

Key words

Mitochondrial plasmid Protoplast cell fusion Lentinula edodes 


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Copyright information

© The Japan Wood Research Society 2006

Authors and Affiliations

  • Masaki Fukuda
    • 1
    Email author
  • Izumi Sendo
    • 1
  • Satoko Imahori
    • 1
  • Yukitaka Fukumasa-Nakai
    • 2
  1. 1.Faculty of AgricultureShinshu UniversityMinamiminowa, NaganoJapan
  2. 2.Tottori Mycological InstituteTottoriJapan

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