Journal of Wood Science

, Volume 51, Issue 5, pp 514–519 | Cite as

Termite repellent sesquiterpenoids from Callitris glaucophylla heartwood

  • Yasutaka Watanabe
  • Rie Mihara
  • Tohru Mitsunaga
  • Tsuyoshi Yoshimura
ORIGINAL ARTICLE

Abstract

Fractions of methanol and ethanol extracts from the heartwood of white cypress pine (Callitris glaucophylla Thompson et Johnson) were investigated for their repellent activity against subterranean termite Coptotermes formosanus Shiraki worker using a two-choice semicircular filter paper test at 0.5% (w/w) concentration. Fraction CY-E2 composed of (−)-citronellic acid, guaiol, α-, β-, and γ-eudesmol isomers as well as an unknown compound, showed the highest statistically significant repellency (97.8% ± 2.2 SEM) of all fractions tested. Bioactivity-guided fractionations using high-performance liquid chromatography led to the isolation of two, oxygenated eudesmane-type sesquiterpenes with α-methylene moieties, both termite-repellent compounds. These compounds were subsequently identified as ilicic acid methyl ester (IAME) and costic acid by means of spectroscopic analyses, electron impact mass spectrometry, and nuclear magnetic resonance spectroscopy. We report the isolation of both IAME and costic acid from C. glaucophylla heartwood for the first time.

Key words

Callitris glaucophylla Termite repellent activity Sesquiterpene Ilicic acid methyl ester Costic acid 

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Copyright information

© The Japan Wood Research Society 2005

Authors and Affiliations

  • Yasutaka Watanabe
    • 1
  • Rie Mihara
    • 1
  • Tohru Mitsunaga
    • 1
  • Tsuyoshi Yoshimura
    • 2
  1. 1.Faculty of BioresourcesMie UniversityTsuJapan
  2. 2.Research Institute for Sustainable HumanosphereKyoto UniversityUjiJapan

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