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Different cortical excitability profiles in hereditary brain iron and copper accumulation

  • Raffaele DubbiosoEmail author
  • Lucia Ruggiero
  • Marcello Esposito
  • Paola Tarantino
  • Marcello De Angelis
  • Francesco Aruta
  • Sabina Pappatà
  • Lorenzo Ugga
  • Alberto Piperno
  • Raffaele Iorio
  • Lucio Santoro
  • Rosa Iodice
  • Fiore Manganelli
Original Article

Abstract

Background and aim

Neurodegeneration with brain iron accumulation (NBIA) and Wilson’s disease (WD) is considered the prototype of neurodegenerative disorders characterised by the overloading of iron and copper in the central nervous system. Growing evidence has unveiled the involvement of these metals in brain cortical neurotransmission. Aim of this study was to assess cortical excitability profile due to copper and iron overload.

Methods

Three patients affected by NBIA, namely two patients with a recessive hereditary parkinsonism (PARK9) and one patient with aceruloplasminemia and 7 patients with neurological WD underwent transcranial magnetic stimulation (TMS) protocols to assess cortical excitability. Specifically, we evaluated the motor thresholds that reflect membrane excitability related to the voltage-gated sodium channels in the neurons of the motor system and the ease of activation of motor cortex via glutamatergic networks, and ad hoc TMS protocols to probe inhibitory-GABAergic (short interval intracortical inhibition, SICI; short-latency afferent inhibition, SAI; cortical silent period, CSP) and excitatory intracortical circuitry (intracortical facilitation, ICF).

Results

Patients with NBIA exhibited an abnormal prolongation of CSP respect to HC and WD patients. On the contrary, neurological WD displayed higher motor thresholds and reduced CSP and SICI.

Conclusion

Hereditary conditions due to overload of copper and iron exhibited peculiar cortical excitability profiles that can help during differential diagnosis between these conditions. Moreover, such results can give us more clues about the role of metals in acquired neurodegenerative disorders, such as Parkinson disease, Alzheimer disease, and multiple sclerosis.

Keywords

Neurodegeneration Metals Wilson’s disease NBIA TMS GABA 

Abbreviations

TMS

Transcranial magnetic stimulation

SAI

Short-latency afferent inhibition

SICI

Short interval intracortical inhibition

RMT

Resting motor threshold

AMT

Active motor threshold

ICF

Intracortical facilitation

CSP

Cortical silent period

MRI

Magnetic resonance imaging

PET

Positron emission tomography

Notes

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

The protocol was approved by the local ethics committee, and the research was conducted in accordance with the 1964 Declaration of Helsinki.

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Copyright information

© Fondazione Società Italiana di Neurologia 2019

Authors and Affiliations

  • Raffaele Dubbioso
    • 1
    Email author
  • Lucia Ruggiero
    • 1
  • Marcello Esposito
    • 1
  • Paola Tarantino
    • 1
  • Marcello De Angelis
    • 1
  • Francesco Aruta
    • 1
  • Sabina Pappatà
    • 2
  • Lorenzo Ugga
    • 3
  • Alberto Piperno
    • 4
  • Raffaele Iorio
    • 5
  • Lucio Santoro
    • 1
  • Rosa Iodice
    • 1
  • Fiore Manganelli
    • 1
  1. 1.Department of Neurosciences, Reproductive Sciences and OdontostomatologyUniversity of Naples “Federico II”NaplesItaly
  2. 2.Institute of Biostructure and BioimagingNational Council of ResearchNapoliItaly
  3. 3.Department of Advanced Biomedical SciencesUniversity of Naples “Federico II”NaplesItaly
  4. 4.Department of Medicine and SurgeryUniversity of Milano-BicoccaMonzaItaly
  5. 5.Department of Translational Medical Sciences, Section of PediatricsUniversity of Naples “Federico II”NaplesItaly

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