Neurological Sciences

, Volume 39, Issue 1, pp 127–133 | Cite as

TLR4 polymorphisms affect stroke risk and inflammatory response in Chinese ischemic stroke patients

  • Lian Gu
  • Jingyan Huang
  • Baoyun Liang
  • Qing Chen
  • Juanjuan Xie
  • Junwei Yang
  • Yan Yan
  • Qianli TangEmail author
Original Article


This study aimed to evaluate the association of the toll-like receptor 4 (TLR4) polymorphisms rs1927914, rs10759932, and rs11536889 with susceptibility to ischemic stroke (IS) and the serum levels of inflammatory cytokines. A total of 816 IS patients and 816 control subjects were genotyped using Sequenom MassARRAY technology. The serum levels of interleukin 1 beta (IL-1β), interleukin 6 (IL-6), interleukin 8 (IL-8), and tumor necrosis factor alpha (TNFα) were measured by enzyme-linked immunosorbent assay. rs1927914 was significantly associated with male IS patients in the additive model [odds ratio (OR) = 0.81; 95% confidence interval (CI) = 0.67–0.99; P = 0.039] and in the allele model (OR = 0.81; 95% CI = 0.66–0.99; P = 0.037). In the dominant model, rs10759932 was significantly associated with the serum TNFα level of the male IS patients [regression coefficient (β) = 0.15; 95% CI = 0.01–0.29; P adj = 0.042]. This polymorphism was also correlated with the serum IL-8 level of female IS patients in the additive model (β = 0.24; 95% CI = 0.25–0.43; P adj = 0.021) and in the recessive model (β = 0.65; 95% CI = 0.11–1.11; P adj = 0.026). The TLR4 gene rs1927914 polymorphism was associated with susceptibility to IS in males. Moreover, the rs10759932 polymorphism may affect inflammatory response in IS patients.


TLR4 Polymorphism Ischemic stroke Inflammatory cytokine 



This work was supported by grants from the National Natural Science Foundation of China (Grant No.81260594); the National Natural Science Foundation of China (Grant No. 81473670); and the Guangxi National Natural Science Foundation (Grant No. 2013GXNSFAA019145).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.


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Copyright information

© Springer-Verlag Italia S.r.l. 2017

Authors and Affiliations

  1. 1.First Affiliated Hospital of Guangxi University of Chinese MedicineNanningChina
  2. 2.Youjiang Medical University for NationalitiesBaisePeople’s Republic of China

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