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Neurological Sciences

, Volume 35, Issue 8, pp 1243–1247 | Cite as

Effects of electromagnetic fields on Reelin and Dab1 expression in the developing cerebral cortex

  • Matin Hemmati
  • Farhad MashayekhiEmail author
  • Fareheh Firouzi
  • Masoumeh Ashori
  • Hamidreza Mashayekhi
Original Article
  • 329 Downloads

Abstract

Many studies describe the biological effects of electromagnetic fields (EMF) including brain damages, neuronal migration and neurogenesis within the central nervous system. Neuronal cell produced in the neuroepithelium migrates along radial glial fibers into the cortical plate. Reelin, which is produced by Cajal–Retzius cells directs neuronal migration. It was shown that Disabled 1 (Dab1) functions downstream of reelin signal transduction pathway that directs the correct cytoarchitecture of the developing cortex. In this study, the EMF effects on total protein concentration (TPC), reelin and Dab1 expression in the developing cortex was studied. 30 pregnant Balb/c mice were separated into three groups: control (n = 10), EMF (n = 10) and SHAM groups (n = 10). The 15-day pregnant mice were placed inside the solenoid for a daily EMF exposure of 5 h for 3 consecutive days (15–17). The SHAM group was also located in the same coil with no exposure. Mice were sacrificed 24 h after the final exposure session. TPC, reelin and Dab1 expression were studied by Bio-Rad protein assay and western blot. No significant change in the TPC was seen in the EMF-treated cerebral cortex samples compared with those from the SHAM and control groups. It was also shown that the reelin and Dab1 expression increases in the EMF-treated cerebral cortex extracts as compared to controls and SHAM group. It is concluded that EMF may play important role in the neural cell migration by increasing reelin and Dab1 expression in the developing cortex.

Keywords

Electromagnetic fields Reelin Dab1 Cerebral cortex Development 

Notes

Acknowledgments

This study was supported by the University of Guilan. The authors thank all people in the Genetics and Developmental Biology laboratories, Department of Biology, Faculty of Sciences, University of Guilan, for their technical assistance.

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Copyright information

© Springer-Verlag Italia 2014

Authors and Affiliations

  • Matin Hemmati
    • 1
  • Farhad Mashayekhi
    • 1
    Email author
  • Fareheh Firouzi
    • 1
  • Masoumeh Ashori
    • 1
  • Hamidreza Mashayekhi
    • 2
  1. 1.Department of Biology, Faculty of SciencesUniversity of GuilanRashtIran
  2. 2.Department of Physics, Faculty of SciencesUniversity of GuilanRashtIran

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