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Neurological Sciences

, Volume 34, Issue 7, pp 1173–1180 | Cite as

Increased expression of ferritin in cerebral cortex after human traumatic brain injury

  • Huan-Dong Liu
  • Wei Li
  • Zhen-Rui Chen
  • Meng-Liang Zhou
  • Zong Zhuang
  • Ding-Ding Zhang
  • Lin Zhu
  • Chun-Hua HangEmail author
Original Article

Abstract

Despite numerous researches and improvements in the past few years, the precise mechanisms underlying secondary brain injury after trauma remain obscure. Iron is essential for almost all types of cells, including nerve cells. However, excess of iron has been proved to contribute to the brain injury following trauma in animal models. As a key iron-handling protein in the brain, ferritin might be involved in iron-induced pathophysiological process of various brain disorders. Therefore, the current study was aimed to investigate the expression of ferritin in the human contused brain. Nineteen contused brain samples were obtained from 19 patients undergoing surgery for brain contusions 3 h–17 d after trauma, and three normal temporal pole samples from 3 patients with petroclival meningioma were collected as controls. Expression of ferritin-H-chain was measured by quantitative real-time polymerase chain reaction (PCR), western blot and immunohistochemistry, respectively. Perl’s reaction was taken for iron staining. The results showed that human traumatic brain injury (TBI) could up-regulate ferritin-H-chain in pericontusional cortex. A marked increase of ferritin was detected in the early group (≤12 h), and remained elevated for a long time till after 48 h post-injury. The location of ferritin-H-chain was found mainly at the neuron-like cells and seldom at glia-like cells. Perl’s reaction showed that most of the iron-positive cells were glia-like cells. These findings suggested that iron and ferritin might be involved in the secondary brain injury and could be therapeutic targets for patients with TBI.

Keywords

Ferritin Iron Traumatic brain injury Inflammation 

Notes

Acknowledgments

This study was supported by grants from National Natural Science Foundation, China (No. 81171170 for C.-H. Hang, No. 8100053 for M.-L. Zhou and No. 81271377 for L. Zhu) and Nature Science Foundation of Jiangsu Province, China (BK2010459).

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Copyright information

© Springer-Verlag Italia 2012

Authors and Affiliations

  • Huan-Dong Liu
    • 1
  • Wei Li
    • 2
  • Zhen-Rui Chen
    • 1
  • Meng-Liang Zhou
    • 2
  • Zong Zhuang
    • 2
  • Ding-Ding Zhang
    • 2
  • Lin Zhu
    • 2
  • Chun-Hua Hang
    • 1
    • 2
    Email author
  1. 1.Department of NeurosurgerySchool of Medicine, Southern Medical University (Guangzhou), Jinling HospitalNanjingPeople’s Republic of China
  2. 2.Department of NeurosurgeryJinling Hospital, School of Medicine, Nanjing UniversityNanjingPeople’s Republic of China

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