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Neurological Sciences

, Volume 32, Issue 3, pp 369–374 | Cite as

Electroacupuncture enhances cell proliferation and neuronal differentiation in young rat brains

  • Jianfeng Gao
  • Sheng Wang
  • Xiaoyang Wang
  • Changlian Zhu
Original Article

Abstract

To investigate the effect of electroacupuncture on cell proliferation and differentiation in young rat hippocampus, postnatal day-14 rats were assigned randomly to control, ketamine anesthesia, nonacupoint, or acupoint electroacupuncture groups. Electroacupuncture was applied at bilateral acupoints (Quchi, Waiguan, Huantiao, and Zusanli) 30 min daily for 7 successive days. The proliferation and neuronal differentiation of proliferated surviving cells in the dentate gyrus were evaluated at 4 weeks after last stimulation. The stimulatory effect of electroacupuncture on cell proliferation had a long-lasting effect, as indicated by the increased phosphor-histone H3-positive cells. The number of proliferated and survival cells, indicated by BrdU labeling, was highest in the electroacupuncture group. The number of newly differentiated neurons, as indicated by BrdU/NeuN double labeling, was significantly higher in the electroacupuncture group than in any of the other groups. This finding provides a theoretical basis for the clinical application of acupuncture to cerebral injury rehabilitation in children.

Keywords

Cell proliferation Electroacupuncture Hippocampus Neurogenesis 

Notes

Acknowledgments

This work was supported by the Medical Science Academy of Henan, a Grant from the Department of Education of Henan Province, the National Natural Science Foundation of China, and the Ministry of Education of China (the third period 211 project).

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Copyright information

© Springer-Verlag 2010

Authors and Affiliations

  • Jianfeng Gao
    • 1
    • 3
  • Sheng Wang
    • 1
  • Xiaoyang Wang
    • 1
    • 2
  • Changlian Zhu
    • 1
    • 2
  1. 1.Department of PediatricsThe Third Affiliated Hospital of Zhengzhou UniversityZhengzhouChina
  2. 2.Henan Key Laboratory for Neonatal Brain InjuryZhengzhouChina
  3. 3.Department of PhysiologyHenan Traditional Chinese Medical CollegeZhengzhouChina

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