Neurological Sciences

, Volume 32, Issue 1, pp 41–51 | Cite as

The effect of lentivirus-mediated TH and GDNF genetic engineering mesenchymal stem cells on Parkinson’s disease rat model

Original Article

Abstract

This study was designed to assess the potential therapeutic efficacy of gene-modified mesenchymal stem cells (MSCs), MSCs-TH and MSCs-GDNF, in PD rats. Fifty-nine PD rat models were divided into five groups and then the gene-modified MSCs were transplanted into the striatum of rats according to the design. Apomorphine-induced rotational behavior in rats was observed weekly; rats which received both MSCs-TH and MSCs-GDNF showed the most significant improvement compared with those in other groups (P < 0.01). Three weeks later, immunohistochemistry analysis found TH-positive cells and GDNF-positive cells in striatal. Eight weeks later, PD rats were killed. HPLC and ELISA results showed DA and GDNF content in striatum of rats which received both MSCs-TH, and MSCs-GDNF was considerably higher compared with those of other groups (P < 0.01),respectively. In conclusion, our results suggest that combined transplantation of MSCs expressing TH and GDNF can lead to remarkable therapeutic effects in a rat model of PD.

Keywords

Mesenchymal stem cells TH gene GDNF gene Parkinson’s disease Gene transfection 

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Copyright information

© Springer-Verlag 2010

Authors and Affiliations

  1. 1.Department of NeurosurgeryJiangsu Provincial Hospital of Traditional Chinese MedicineNanjingPeople’s Republic of China
  2. 2.Department of NeurosurgeryThe First Affiliated Hospital of Nanjing Medical UniversityNanjingPeople’s Republic of China
  3. 3.Department of SurgeryThe First Affiliated Hospital of Nanjing Medical UniversityNanjingPeople’s Republic of China

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