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Neurological Sciences

, Volume 32, Issue 2, pp 221–228 | Cite as

Proteomics analysis of MPP+-induced apoptosis in SH-SY5Y cells

  • Hongrong Xie
  • Ming Chang
  • Xinyu Hu
  • Danping Wang
  • Mingxiu Tian
  • Guoyi Li
  • Huiyi Jiang
  • Ying Wang
  • Zhong Dong
  • Yuhua Zhang
  • Linsen Hu
Original Article

Abstract

Accumulating evidence suggests that oxidative stress plays a pivotal role in dopaminergic neurodegeneration. However, the kinds of proteins involved in the response to oxidative stress remain unclear. In the present study, SH-SY5Y cells were treated with neurotoxin 1-methyl-4-phenyl-pyridinium ion (MPP+) to induce apoptotic neuronal injury. 2D-DIGE followed by MALDI-TOF-MS was used to determine the changing protein levels. Proteomics analysis revealed that 22 proteins were differentially altered in MPP+-treated SH-SY5Y cells, of which 7 were up-regulated proteins and 15 were down-regulated proteins, respectively. Three protein spots were unambiguously identified as sorcin, annexin V, and ribosomal protein P0. The three proteins showed a significant increase in level, suggesting a role in MPP+-induced apoptosis. The functional roles of these three proteins collectively indicate that multiple mechanisms are pertinent in the underlying pathogenesis of Parkinson’s disease (PD), such as apoptosis, calcium homeostasis, and DNA insults.

Keywords

Proteomics SH-SY5Y cells MPP+ Sorcin Annexin V Ribosomal protein P0 

Notes

Acknowledgments

This work was supported by a grant from the Distinguished Professor Foundation of Jilin University to Dr. Linsen Hu (450011011204).

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Copyright information

© Springer-Verlag 2010

Authors and Affiliations

  • Hongrong Xie
    • 1
    • 3
  • Ming Chang
    • 1
  • Xinyu Hu
    • 2
  • Danping Wang
    • 1
  • Mingxiu Tian
    • 1
  • Guoyi Li
    • 1
  • Huiyi Jiang
    • 1
  • Ying Wang
    • 1
  • Zhong Dong
    • 1
  • Yuhua Zhang
    • 3
  • Linsen Hu
    • 1
  1. 1.Department of Neurology, The First HospitalJilin UniversityChangchunPeople’s Republic of China
  2. 2.The Second HospitalJilin UniversityChangchunChina
  3. 3.Department of Computerized TomographyDaqing Oilfield General HospitalDaqingChina

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