Neurological Sciences

, Volume 31, Issue 3, pp 283–291 | Cite as

CSF proteomic analysis in patients with normal pressure hydrocephalus selected for the shunt: CSF biomarkers of response to surgical treatment

  • Antonio Scollato
  • Alessandro Terreni
  • Anna Caldini
  • Benedetta Salvadori
  • Pasquale Gallina
  • Simona Francese
  • Guido Mastrobuoni
  • Giuseppe Pieraccini
  • Gloriano Moneti
  • Luca Bini
  • Gianni Messeri
  • Nicola Di Lorenzo
Original Article


The aim of our pilot study was to investigate, by a proteomic approach, the expressed differences in cerebrospinal fluid (CSF) protein patterns in order to aid in the diagnosis and treatment of normal pressure hydrocephalus (NPH). Seventeen patients with NPH, selected by Intracranial-Pressure monitoring (ICPmo), underwent implantation of a shunt and after 6 months were clinically re-evaluated. Thirteen patients improved, whereas four did not. During ICPmo CSF was collected and its proteoma was analyzed by 2D gel electrophoresis and mass spectrometry. The over-expression of α2HS glycoprotein, α1 antichimotrypsin and α1beta glycoprotein and the under-expression of glial fibrillary acidic protein, apolipoproteins (AIV, J and E), complement C3c, anti-thrombin, α2 antiplasmin and albumin seem to be associated with a positive response to surgery. Most of these proteins have been reported to be altered in Alzheimer disease, supporting the hypothesis of a possible link between these two nosological entities.


Normal pressure hydrocephalus Cerebrospinal fluid Proteoma 2D-electrophoresis 


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Copyright information

© Springer-Verlag 2009

Authors and Affiliations

  • Antonio Scollato
    • 1
  • Alessandro Terreni
    • 2
  • Anna Caldini
    • 2
  • Benedetta Salvadori
    • 2
  • Pasquale Gallina
    • 1
  • Simona Francese
    • 3
  • Guido Mastrobuoni
    • 3
  • Giuseppe Pieraccini
    • 3
  • Gloriano Moneti
    • 3
  • Luca Bini
    • 4
  • Gianni Messeri
    • 2
  • Nicola Di Lorenzo
    • 1
  1. 1.Department of NeurosurgeryUniversity of FlorenceFlorenceItaly
  2. 2.Department of Laboratory DiagnosticCareggi University Hospital of FlorenceFlorenceItaly
  3. 3.Mass Spectrometry Center of the University of FlorenceFlorenceItaly
  4. 4.Functional Proteomics Laboratory, Department of Molecular BiologyUniversity of SienaSienaItaly

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