Neurological Sciences

, Volume 27, Issue 5, pp 312–316

Effect of nitric oxide on lymphocytes from sporadic amyotrophic lateral sclerosis patients: toxic or protective role?

  • C. Cereda
  • E. Cova
  • C. Di Poto
  • A. Galli
  • G. Mazzini
  • M. Corato
  • M. Ceroni
ORIGINAL

DOI: 10.1007/s10072-006-0702-z

Cite this article as:
Cereda, C., Cova, E., Di Poto, C. et al. Neurol Sci (2006) 27: 312. doi:10.1007/s10072-006-0702-z

Abstract

Markers of oxidative and nitrosative stress have been found in spinal cord, cortex, cerebrospinal fluid and plasma of patients affected by amyotrophic lateral sclerosis (ALS), a fatal disorder characterised by progressive motor neuron degeneration. In this study, we investigated the effect of the NO-releasing agent, diethylamine NONOate (NONO), on lymphocytes from patients affected by the sporadic form of ALS (SALS) and controls by flow cytometry. In the same experimental conditions we investigated the expression of the antioxidant proteins, Bcl-2 and SOD1. Incubation with NONO induced cell damage in control lymphocytes but did not further damage the already affected untreated SALS lymphocytes. The incubation with NONO induced a time-dependent decrease of Bcl-2 and SOD1 in control lymphocytes. Surprisingly, in SALS lymphocytes the NONO treatment increased the expression of these proteins, which in basal conditions was depressed compared to control lymphocytes.

Key words

Nitric oxide Amyotrophic lateral sclerosis Lymphocytes Oxidative stress Bcl-2 SOD1 

Copyright information

© Springer-Verlag Italia 2006

Authors and Affiliations

  • C. Cereda
    • 1
  • E. Cova
    • 1
  • C. Di Poto
    • 1
  • A. Galli
    • 2
  • G. Mazzini
    • 3
  • M. Corato
    • 1
  • M. Ceroni
    • 1
    • 2
    • 4
  1. 1.Laboratory of Experimental NeurobiologyI.R.C.C.S. Foundation “C. Mondino” Institute of Neurology, Centro RicerchePaviaItaly
  2. 2.Department of NeurologyPoliclinico of MonzaMonzaItaly
  3. 3.IGM-CNR, Histochemistry and Cytometry, Department of Animal BiologyUniversity of PaviaPaviaItaly
  4. 4.Department of NeurosciencesUniversity of PaviaPaviaItaly

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