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Serial reversal learning in freshwater stingrays (Potamotrygon motoro)

  • Martha M. M. Daniel
  • Vera SchluesselEmail author
Original Paper
First Online:

Abstract

Serial reversal learning is considered a reliable approach for the testing of behavioral flexibility, and animals that inhabit fluctuating habitats and different environments are expected to possess behavioral and cognitive flexibility. The ocellate river stingray (Potamotrygon motoro) is one such species. Comprising the first serial reversal learning experiment among elasmobranchs, this study trained seven juvenile P. motoro in a visual two-alternative forced-choice task, in which a food-rewarded stimulus and an unrewarded alternative stimulus were presented in pseudo-random order on either side of a barrier. In the session after a stingray reached the learning criterion (LC), food was associated with the alternative stimulus, and this reward association continued to be switched whenever LC was achieved. Overall, five stingrays reversed successfully at least once. All of them required more sessions (mean = 67.8 sessions) for the first reversal than they needed during training (mean = 22 sessions). One stingray demonstrated progressive improvement across four reversal phases, thus showing that Potamotrygon motoro can inhibit a previously learned association faster with experience, probably as it develops strategies for rule identification. One individual became slower to reach the (LC) across three reversal phases, which may indicate a dominant influence of proactive interference. Another stingray demonstrated large fluctuations across three reversals. The fourth individual completed two reversals and required approximately the same number of sessions for each. One stingray had just completed one reversal by the end of this study. Since stingrays took longer than a related species to reverse learning, future studies could look at the effects of changing stimulus type or training regime on the performance of P. motoro.

Keywords

Behavior Cognition Progressive improvement Vision Elasmobranch Learning 
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Notes

Acknowledgements

We would like to sincerely thank Slawa Braun (animal caretaker, Institute of Zoology, University Bonn), for his availability regarding maintenance questions, repairs, and animal illness; Dr. Werner Ekau (Leibniz Center for Tropical Marine Research) for his insight and advice regarding statistics; and Dr. Ingolf Rick (Institute for Evolutionary Biology and Ecology), for helping obtain the visual spectra of the experimental stimuli.

Funding

No funding was provided for his study.

Compliance with ethical standards

Conflict of interest

Author Vera Schluessel declares that she has no conflict of interest. Author Martha M.M. Daniel declares that she has no conflict of interest.

Ethical approval

All applicable international, national, and/or institutional guidelines for the care and use of animals were followed.

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© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Institute of ZoologyRheinische Friedrich-Wilhelms-Universität BonnBonnGermany

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