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Evaluation of 3M™ loop-mediated isothermal amplification-based kit and 3M™ ready-to-use plating system for detection of Listeria in naturally contaminated leafy vegetables, chicken, and their related processing environments

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Abstract

The effectiveness of two different rapid methods involving the 3M™ molecular detection assay Listeria and the 3M™ Petrifilm environmental Listeria Plate were evaluated for the rapid detection of Listeria from naturally contaminated vegetables and chicken-processing environments against the standard culture-based method. A total of 178 samples were examined for the presence of Listeria. A total of 47/178 (26.4%) by standard ISO culture-based method (EN ISO 11290-1), 42/178 (23.6%) by 3M™ MDA Listeria and 40/178 (22.5%) by 3M™ Petrifilm EL Plate showed positive results, respectively. The accuracy, sensitivity, specificity, positive predictive value, and negative predictive value for 3M™ MDA Listeria and 3M™ Petrifilm EL Plate were 97.2, 89.4, 99.3, 97.7, 96.4% and 96.1, 85.1, 100.0, 100.0, 94.9%, respectively. Based on the Cohen’s Kappa value, there was a complete and robust concordance between 3M™ MDA Listeria (0.911) and 3M™ Petrifilm EL Plates (0.894) as compared to the standard culture-based method.

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Acknowledgements

The first author is thankful to USM-IPS for providing a USM Global fellowship. This work was supported by the Universiti Sains Malaysia, with a University Research Grants (1001/PTEKIND/811289).

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Authors and Affiliations

  1. Food Technology Division, School of Industrial Technology, Universiti Sains Malaysia, 11800, Minden, Penang, Malaysia

    Mustapha Goni Abatcha, Pei Ling Tan, Li- Oon Chuah, Gulam Rusul & Mohd Esah Effarizah

  2. Food Safety Department, 3M Malaysia, Level 8, Block F, Oasis Square, Ara Damansara, 47301, Petaling Jaya, Selangor, Malaysia

    S. R. Chandraprasad

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  1. Mustapha Goni Abatcha
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  2. Pei Ling Tan
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  3. Li- Oon Chuah
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  4. Gulam Rusul
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  5. S. R. Chandraprasad
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  6. Mohd Esah Effarizah
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Correspondence to Mohd Esah Effarizah.

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Abatcha, M., Tan, P.L., Chuah, L.O. et al. Evaluation of 3M™ loop-mediated isothermal amplification-based kit and 3M™ ready-to-use plating system for detection of Listeria in naturally contaminated leafy vegetables, chicken, and their related processing environments. Food Sci Biotechnol 29, 1141–1148 (2020). https://doi.org/10.1007/s10068-020-00762-2

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