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Effect of subcritical water extraction conditions on the activity of alcohol metabolizing enzymes, ACE inhibition, and tyrosinase inhibition in Protaetia brevitarsis larvae

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In order to develop processing methods with high physiological activity for Protaetia brevitarsis larvae (PBL), subcritical water (SCW) extraction was applied. The dried powder (1 g) of PBL was extracted with 10 mL distilled water at 100, 200, and 300 °C for 30 min. The SCW treatment significantly (p < 0.05) increased some physiological activities of the PBL extracts. The SCW extract at 300 °C increased alcohol dehydrogenase, acetaldehyde dehydrogenase, and tyrosinase inhibitory activities from 192.3 ± 4.1% to 452.2 ± 0.5%, 125.4 ± 2.9% to 153.3 ± 0.4%, and − 7.0 ± 0.7% to 26.1 ± 1.4%, respectively, compared to the extract at 100 °C. Contrarily, the inhibition activity of angiotensin converting enzyme was the highest at 200 °C. These results suggest that SCW is a suitable method to extract and maintain the physiological activity of PBL.

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  1. Bergmeyer HU. Alcohol dehydrogenase. Vol 1, pp. 428-429. In: Methods of Enzymatic Analysis. Bergmeyer HU (ed). Academic Press, New York, NY (1974)

  2. Carr AG, Mammucari R, Foster NR. A review of subcritical water as a solvent and its utilisation for the processing of hydrophobic organic compounds. Chem. Eng. J. 172: 1-17 (2011)

  3. Choi IH, Yu R, Lim YJ, Choi GS, Choi SU, Hwang JI, Son JS, Chung TH. Antithrombotic efficacy of Protaetia brevitarsis extract. J. Enviorn. Sci. Int. 28: 639-643 (2019)

  4. Chon JW, Kweon H, Jo YY, Yeo JH, Lee HS. Protective effects of extracts of Protaetia brevitarsis on carbon tetrachloride-induced hepatotoxicity in the mice. J. Seric. Entomol. Sci. 50: 93-100 (2012)

  5. Chung MY, Hwang JS, Goo TW, Yun EY. Analysis of general composition and harmful material of Protaelia brevitarsis. J. Life Sci. 23: 664-668 (2013)

  6. Cushman DW, Cheung HS. Spectrophotometric assay and properties of the angiotensin-converting enzyme of rabbit lung. Biochem. Pharmacol. 20: 1637-1648 (1971)

  7. Fujita H, Yokoyama K, Yoshikawa M. Classification and antihypertensive activity of angiotensin I-converting enzyme inhibitory peptides derived from food proteins. J. Food Sci. 65: 564-569 (2000)

  8. Han JK, Sung SC, Jo MJ, Lee SC. Antioxidant, ACE inhibitory, and acetylcholinesterase inhibitory activities of subcritical water extract of blue mussel. Food Sci. Biotenol. 27: 847-851 (2018)

  9. Jo EK, Heo DJ, Kim JH, Lee YH, Ju YC, Lee SC. The effects of subcritical water treatment on antioxidant activity of golden oyster mushroom. Food Bioprocess Technol. 6: 2555-2561 (2013)

  10. Jo MJ, Han JK, Sung SC, Lee SC. Preparation of subcritical water extract with improved physiological activity of Styela clava. J. Korean Soc. Food Sci. Nutr. 47: 1112-1117 (2018)

  11. Kim SJ, Matsushita Y, Fukushima K, Aoki D, Yagami S, Yuk HG, Lee SC. Antioxidant activity of a hydrothermal extract from watermelons. LWT-Food Sci. Technol. 59: 361-368 (2014a)

  12. Kim SK, Ok DL, Park E, Lee SC. Effects of hot water extracts of domestic blue mussel and New Zealand green lipped mussel on alcohol metabolizing enzymatic, DPPH radical scavenging, and angiotensin converting enzyme inhibitory activities. J. Korean Soc. Food Sci. Nutr. 43: 1363-1368 (2014b)

  13. Kim YJ, Uyama H. Tyrosinase inhibitors from natural and synthetic sources: structure, inhibitory mechanism and perspective for the future. Cell Mol. Life Sci. 62: 1707-1723 (2005)

  14. Koivula T, Koivusalo M. Different forms of rat liver aldehyde dehydrogenase and their subcellular distribution. Biochim. Biophys. Acta 397: 9-23 (1975)

  15. Korean Food Table. Available from: Accessed Sept. 30, 2019.

  16. Kus NS. Organic reactions in subcritical and supercritical water. Tetrahedron 68: 949-958 (2012)

  17. Kwon EY, Yoo J, Yoon YI, Hwang JS, Goo TW, Kim MA, Choi YC, Yu EY. Pre-treatment of the white-spotted flower chafer (Protaetia brevitarsis) as an ingredient for novel foods. J. Korean Soc. Food Sci. Nutr. 42: 397-402 (2013)

  18. Lee HJ, Seo M, Lee JH, Kim IW, Kim SY, Hwang JS, Kim MA. Inhibitory effect of Protaetia brevitarsis seulensis ethanol extract on neuroinflammation in LPS-stimulated BV-2 microglia. J. Life Sci. 29: 1096-1103 (2019)

  19. Lee HS, Ryu HJ, Song HJ, Lee SO. Enzymatic preparation and antioxidant activities of protein hydrolysates from Protaetia brevitarsis larvae. J. Korean Soc. Food Sci. Nutr. 46: 1164-1170 (2017)

  20. Lee SY, Hur SJ. Antihypertensive peptides from animal products, marine organisms, and plants. Food Chem. 228: 506-517 (2017)

  21. Lee YD. Properties of aqueous extract of Protaetia brevitarsis larva and mountain ginseng fermented by Lactobacillus brevis. J. Food Hyg. Saf. 33: 369-374 (2018)

  22. Liber CS. Hepatic metabolic and toxic effects of ethanol. Alcohol Clin. Exp. Res. 15: 573-592 (1991)

  23. Sim SY, Ahn HY, Seo KI, Cho YS. Physiological properties and biological activities of Protaetia brevitarsis seulensis larvae fermented by several kinds of micro-organisms. J. Life Sci. 28: 827-834 (2018)

  24. Sugano T, Handler JA, Yoshihara H, Kizaki Z, Thurman RG. Acute and chronic ethanol treatment in vivo increases malate-aspartate shuttle capacity in perfused rat liver. J. Biol. Chem. 265: 21549-21553 (1990)

  25. Sung GA, Kim MH, Park SN. Anti-inflammatory and whitening effects of Protaetia brevitarsis Seulensis extracts by oriental conversion methods. J. Soc. Cosmet. Sci. Korea. 42: 421-432 (2016)

  26. van Huis A, Itterbeeck JV, Klunder H, Mertens E, Halloran A, Muir G, Vantomme P. Edible insects: future prospects for food and feed security. Food and Agriculture Organization of the United Nations, Rome (2013)

  27. Vanni A, Gastaldi D, Giunta G. Kinetic investigations on the double enzymic activity of the tyrosinase mushroom. Ann. Chim. Rome 80: 35-60 (1990)

  28. Yun EY, Hwang JS. Status and prospect for development of insect foods. Food Sci. Ind. 49: 31-39 (2016)

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This work was supported by Kyungnam University Foundation Grant, 2019.

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Correspondence to Seung-Cheol Lee.

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Bae, S., Lee, S. Effect of subcritical water extraction conditions on the activity of alcohol metabolizing enzymes, ACE inhibition, and tyrosinase inhibition in Protaetia brevitarsis larvae. Food Sci Biotechnol (2020).

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  • Protaetia brevitarsis larvae
  • Subcritical water
  • Alcohol metabolizing enzymes
  • ACE inhibitory activity
  • Tyrosinase inhibitory activity