Food Science and Biotechnology

, Volume 28, Issue 6, pp 1669–1677 | Cite as

High-performance liquid chromatography and gas chromatography to set the analysis method of stearoyl lactylate, a food emulsifier

  • Juhee Park
  • Hyondeog Kim
  • Seungran Hong
  • Hee-Jae Suh
  • Chan LeeEmail author


Stearoyl lactylates (SLs, E481/E482) and their sodium and calcium salts are emulsifiers commonly used as food additives. This study was performed to set the analytical method of SLs in Korea using high-performance liquid chromatography (HPLC) and gas chromatography (GC). A new HPLC method using C18 column with water and methanol as the eluents, and a new GC method using DB-1 column with He as the carrier gas, were developed and were compared with previously reported analytical methods. The new HPLC and GC methods indicated a coefficient of determination (r2) of 0.999 in the calibration curves. The detection and quantification limits are 0.26 and 0.78 μg/kg for the HPLC method, respectively, and 16.54 and 50.12 μg/kg for the GC method, respectively. The new HPLC and GC methods indicated precision of 0–2.0%, and accuracy of 92.7–108.5%. Therefore, they can be applied in numerous food samples to determine the level of SLs.


Stearoyl lactylates High-performance liquid chromatography (HPLC) analysis Gas chromatography-flame ionization detector analysis Sodium stearoyl lactylate Calcium stearoyl lactylate 



This research was supported by a Grant (17162MFDS021) from the Ministry of Food and Drug Safety in 2017. This research was also supported by the Chung-Ang University Graduate Research Scholarship in 2017.


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Copyright information

© The Korean Society of Food Science and Technology 2019

Authors and Affiliations

  • Juhee Park
    • 1
  • Hyondeog Kim
    • 1
  • Seungran Hong
    • 1
  • Hee-Jae Suh
    • 2
  • Chan Lee
    • 1
    Email author
  1. 1.Advanced Food Safety Research Group, BrainKorea21 Plus, Department of Food Science and TechnologyChung-Ang UniversityAnseongRepublic of Korea
  2. 2.Department of Food ScienceSun Moon UniversityAsan-SiRepublic of Korea

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