Anti-inflammatory, anti-oxidative stress effect of Phascolosoma esculenta oligosaccharides on Escherichia coli-induced sepsis mice
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Bacterial infection is the most common cause of sepsis. In this study, Phascolosoma esculenta oligosaccharides (PEOs) were prepared to evaluate their resistance against E. coli-induced sepsis. HPLC–MS and FT-IR indicated that PEOs were composed of d-glucosyl, d-galactosyl, with small amount of d-mannosyl, d-arabinosyl and residues with α- and β-type linkage. Different dosage administrations of PEOs for 30 days significantly improved ICR mice survival rate and bacterial clearance ability (P < 0.01) after as E. coli injection. Moreover, PEOs significantly reduced the secretion of IL-1β and TNF-α and enhanced that of IL-10 in sepsis mice, enhanced the antioxidant enzyme activities and total antioxidant capacity, decreased MDA level in the serum, and upregulated mRNA expression of Nrf2 (P < 0.01). All these results indicate that PEOs could improve the resistance of ICR mice against E. coli-induced sepsis that attributed to anti-inflammatory and anti-oxidative stress.
KeywordsPhascolosoma esculenta oligosaccharides Sepsis Oxidative stress Inflammation
This work was supported by the National Natural Science Foundation of China (31601476), Zhejiang Provincial Natural Science Foundation of China (LQ15C200002), and sponsored by K. C. Wong Magna Fund in Ningbo University.
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Conflict of interest
The authors declare no conflict of interest.
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