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Food Science and Biotechnology

, Volume 28, Issue 6, pp 1819–1828 | Cite as

Red ginseng extract regulates differentiation of monocytes to macrophage and inflammatory signalings in human monocytes

  • Bobin Kang
  • Chae Young Kim
  • Jisu Hwang
  • Sojung Sun
  • Hyunwon Yang
  • Hyung Joo Suh
  • Hyeon-Son ChoiEmail author
Article

Abstract

This study was aimed to investigate the effect of red ginseng extract (RGE) on monocyte to macrophage differentiation and inflammatory signalings in THP-1 human monocytes. In HPLC analysis, RGE contained saponin level of 516 μg/mg (extract) with 14 ginsenosides. RGE effectively suppressed the monocyte-to-macrophage differentiation induced by phorbol 12-myristate 13-acetated (PMA) by inhibiting the THP-1 cell adhesion. This result is evidenced by the down-regulation of cluster of differentiation molecule β (CD11β) and CD36. RGE significantly reduced translocation of nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) (78%), while cytosolic NF-κB was increased (53%), compared with LPS group. In addition, RGE significantly increased the protein abundance of nuclear factor (erythroid-derived 2)-like 2 (Nrf2) and its target protein, hemoxygenase-1 (HO-1), but, Kelch-like ECH-associated protein 1 (KEAP1), a negative regulator of Nrf2, was greatly decreased by RGE. Furthermore, RGE effectively mediated the regulation of Nrf2 level in nucleus and cytoplasm of THP-1.

Keywords

Human monocyte (THP-1) Red ginseng extract (RGE) Monocyte-to-macrophage differentiation Inflammatory signaling 

Notes

Acknowledgements

This study was supported by a research grant from Seoul Women’s University (2019).

Compliance with ethical standards

Conflict of interest

The authors declare no conflict of interest.

Supplementary material

10068_2019_611_MOESM1_ESM.docx (72 kb)
Supplementary material 1 (DOCX 71 kb)

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Copyright information

© The Korean Society of Food Science and Technology 2019

Authors and Affiliations

  • Bobin Kang
    • 1
  • Chae Young Kim
    • 1
  • Jisu Hwang
    • 1
  • Sojung Sun
    • 2
  • Hyunwon Yang
    • 2
  • Hyung Joo Suh
    • 1
  • Hyeon-Son Choi
    • 3
    Email author
  1. 1.Department of Public Health SciencesKorea UniversitySeoulRepublic of Korea
  2. 2.Department of Bioenvironmental Technology, College of Natural ScienceSeoul Women’s UniversitySeoulRepublic of Korea
  3. 3.Department of Food Science and Technology, College of Natural ScienceSeoul Women’s UniversitySeoulRepublic of Korea

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