Food Science and Biotechnology

, Volume 28, Issue 6, pp 1801–1809 | Cite as

Combination of mineral trioxide aggregate and propolis promotes odontoblastic differentiation of human dental pulp stem cells through ERK signaling pathway

  • Jae-Hwan Kim
  • Soo-Yung Kim
  • Su-Mi Woo
  • Ha-Na Jeong
  • Ji-Yeon Jung
  • Seon-Mi Kim
  • Hae-Soon LimEmail author


The aim of this study is to investigate combined effects of mineral trioxide aggregate (MTA) and propolis on odontoblastic differentiation of human dental pulp stem cells (DPSCs) and to find a signaling pathway involved. Combination of MTA and propolis significantly up-regulated the expression of DSPP and DMP1, and facilitated a mineral nodule formation (p < 0.05). Treatments with MTA, propolis or combined increased the phosphorylation of extracellular signal-regulated kinases (ERK), one of mitogen-activated protein kinases signaling cascades during odontogenic differentiation of DPSCs (p < 0.05), and U0126, an inhibitor of ERK, decreased calcium deposits (p < 0.05). Combination of MTA and propolis promotes odontogenic differentiation and mineralization of DPSCs through ERK pathway.


ERK signaling Human dental pulp cells Mineral trioxide aggregate Odontoblastic differentiation Propolis 



This research was supported by a Grant (HCRI 16919-1) from Chonnam National University Hwasun Hospital Institute for Biomedical Science.

Compliance with ethical standards

Conflict of interest

The authors report no conflict of interest related to this study.


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Copyright information

© The Korean Society of Food Science and Technology 2019

Authors and Affiliations

  1. 1.Department of Pediatric Dentistry, School of Dentistry, Dental Science Research InstituteChonnam National UniversityGwangjuRepublic of Korea
  2. 2.Department of Oral Physiology, School of Dentistry, Dental Science Research InstituteChonnam National UniversityGwangjuRepublic of Korea
  3. 3.Department of DentistryChonnam National University Hwasun HospitalHwasun-gunRepublic of Korea
  4. 4.Department of Dental Education, School of Dentistry, Dental Science Research InstituteChonnam National UniversityGwangjuRepublic of Korea

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