Improved astaxanthin production by Xanthophyllomyces dendrorhous SK984 with oak leaf extract and inorganic phosphate supplementation
- 17 Downloads
Astaxanthin is widely used in food, feed and nutraceutical industries. Xanthophyllomyces dendrorhous is one of the most promising natural sources of astaxanthin. However, the astaxanthin yield in the wild-type X. dendrorhous is considered low for industrial application. In the present study, X. dendrorhous ATCC 66272 was subjected to two-staged mutagenesis: (i) UV light and (ii) N-methyl-N′-nitro-N-nitroso-guanidine (NTG) toward attaining higher astaxanthin yield. The UV-irradiation mutant, X. dendrorhous SK974 showed 1.7-fold (1.07 mg/g) higher astaxanthin production as compared with the wild-type strain (0.65 mg/g). The UV mutant strain was then treated with NTG, designated as X. dendrorhous SK984, displayed further 1.4-fold (1.45 mg/g) higher astaxanthin production. Furthermore, the oak leaf extract (5%, v/v) and inorganic phosphate (KH2PO4, 3 mM) supplementation resulted about 1.4-fold (1.98 mg/g) higher astaxanthin production as compared with control (1.45 mg/g) in X. dendrorhous SK984. These findings serve as a platform suggesting that intersecting approaches might be aimed toward systematically enhanced astaxanthin production.
KeywordsAstaxanthin Inorganic phosphate Mutagenesis Oak leaf extract X. dendrorhous
This work was supported by the Konkuk University (Seoul, Republic of Korea) in 2018.
- An GH, Schuman, DB, Johnson EA. Isolation of X. dendrorhous mutants with increased astaxanthin content. Appl. Environ. Microbiol. 55: 116–124 (1989)Google Scholar
- Kim SK, Lee JH, Lee CH, Yoon YC. Increased carotenoid production in Xanthophyllomyces dendrorhous G276 using plant extracts. J. Microbiol. 45: 128–132 (2007)Google Scholar
- Lewis MJ, Ragot N, Berlant MC, Miranda M. Selection of astaxanthin-overproducing mutants of Phaffia rhodozyma with β-ionone. Appl. Environ. Microbiol. 56: 2944–2945 (1990)Google Scholar
- Nguyen KD. Astaxanthin: A comparative case of synthetic vs. natural production. Chemical and Biomolecular Engineering Publications and Other Works. http://trace.tennessee.edu/utk_chembiopubs/94 (2013)
- Wolkerstorfer SV, Wonisch A, Stankova T, Tsvetkova N, Tausz M. Seasonal variations of gas exchange, photosynthetic pigments, and antioxidants in Turkey oak (Quercus cerris L.) and Hungarian oak (Quercus frainetto Ten.) of different age. Trees 25: 1043–1052 (2011)Google Scholar