Improved astaxanthin production by Xanthophyllomyces dendrorhous SK984 with oak leaf extract and inorganic phosphate supplementation

  • Damini Kothari
  • Jun-Hyeong Lee
  • Jung-Whan Chon
  • Kun-Ho Seo
  • Soo-Ki KimEmail author


Astaxanthin is widely used in food, feed and nutraceutical industries. Xanthophyllomyces dendrorhous is one of the most promising natural sources of astaxanthin. However, the astaxanthin yield in the wild-type X. dendrorhous is considered low for industrial application. In the present study, X. dendrorhous ATCC 66272 was subjected to two-staged mutagenesis: (i) UV light and (ii) N-methyl-N′-nitro-N-nitroso-guanidine (NTG) toward attaining higher astaxanthin yield. The UV-irradiation mutant, X. dendrorhous SK974 showed 1.7-fold (1.07 mg/g) higher astaxanthin production as compared with the wild-type strain (0.65 mg/g). The UV mutant strain was then treated with NTG, designated as X. dendrorhous SK984, displayed further 1.4-fold (1.45 mg/g) higher astaxanthin production. Furthermore, the oak leaf extract (5%, v/v) and inorganic phosphate (KH2PO4, 3 mM) supplementation resulted about 1.4-fold (1.98 mg/g) higher astaxanthin production as compared with control (1.45 mg/g) in X. dendrorhous SK984. These findings serve as a platform suggesting that intersecting approaches might be aimed toward systematically enhanced astaxanthin production.


Astaxanthin Inorganic phosphate Mutagenesis Oak leaf extract X. dendrorhous 



This work was supported by the Konkuk University (Seoul, Republic of Korea) in 2018.


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Copyright information

© The Korean Society of Food Science and Technology 2019

Authors and Affiliations

  • Damini Kothari
    • 1
  • Jun-Hyeong Lee
    • 1
  • Jung-Whan Chon
    • 1
    • 2
  • Kun-Ho Seo
    • 2
  • Soo-Ki Kim
    • 1
    Email author
  1. 1.Department of Animal Science and TechnologyKonkuk UniversitySeoulRepublic of Korea
  2. 2.KU Center for Food SafetyKonkuk UniversitySeoulRepublic of Korea

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