Simultaneous detection of fruit allergen-coding genes in tomato, apple, peach and kiwi through multiplex PCR
Fruit allergies have become more common in recent years, and are now a serious health problem. In this study, a multiplex PCR assay was used to detect potential fruit allergens causing food allergy labeling in Korea. For the detection of these allergens, specific primer pairs were designed to amplify the allergen-coding genes Cyclophilin (tomato), Mdtl 1 (apple), Pru p 2.01A (peach) and Pectin methylesterase inhibitor (kiwi), and primer pair targeting the 18S ribosomal RNA gene was additionally used as an endogenous control. Primer specificity was assessed with 23 plant species. A mixture of DNA from the four fruits was serially diluted and used to determine the sensitivity of the multiplex PCR assay, which was approximately 0.08 ng. Eleven commercial fruit products were evaluated to verify the applicability of the multiplex PCR assay. This assay is expected to be a specific and efficient method for detecting fruit allergens in foods.
KeywordsFood allergy Fruit allergen Multiplex PCR Peach Tomato Apple Kiwi
This research was supported by Grant 16163MFDS003 from the Ministry of Food and Drug Safety in Korea.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
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