Effect of acid treatment on extraction yield and gel strength of gelatin from whiptail stingray (Dasyatis brevis) skin
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Chemical properties of fish gelatins differ from those of conventional mammalian sources, representing an attractive technological alternative for the food industry. Ray filleting generates a considerable amount of skin waste that can be used as a collagen source for gelatin extraction. Thus, this research evaluated the HCl and CH3COOH effect, at 0.01, 0.025, 0.05, 0.075, 0.1, 0.15, and 0.2 M, on extraction yield, molecular weight distribution, and gel strength (GS) of whiptail stingray (Dasyatis brevis) skin gelatins. Results showed differences (P < 0.05) between acid type and concentration used. CH3COOH (0.15 M) gave the highest extraction yield (7.0% vs. 5.5% at 0.15 M HCl) and GS (653 ± 71 g vs. 619.5 ± 82 g at 0.2 M HCl). Gelatin electrophoretic profile from CH3COOH revealed α-/β-components and high molecular weight (> 200 kDa) polymers. Ray gelatin GS was higher than commercial bovine gelatin, suggesting its possible use for technological food applications.
KeywordsFish skin collagen Fish gelatin Acid treatment Gel strength Extraction yield
Author Marco A. Sántiz-Gómez wishes to thank Consejo Nacional de Ciencia y Tecnología (CONACyT) of the Mexican Republic for the scholarship received during this research.
- AOAC. Official Methods of Analysis of AOAC International, 17th ed. Association of Official Analytical Chemists, Gaithersburg, MD, USA (2000)Google Scholar
- Bama P, Vijayalakshimi M, Jayasimman R, Kalaichelvan PT, Deccaraman M, Sankaranarayanan S. Extraction of collagen from cat fish (Tachysurus macalatus) by pepsin digestion and preparation and characterization of collagen chitosan sheet. Int. J. Pharm. Pharm. Sci. 2(4): 133–137 (2010)Google Scholar
- Boran G, Regenstein JM. Fish gelatin. Vol. 60, pp. 119–143. In: Advances in Food and Nutrition Research. Steve LT (ed). Academic Press, New York, NY, USA (2010)Google Scholar
- GMIA. Standard testing methods for edible gelatin. Available from: http://www.gelatin-gmia.com/images/GMIA_Official_Methods_of_Gelatin_Revised_2013.pdf. Accessed 12 September 2018
- Huss HH. Fresh Fish—Quality and Quality Changes. Food and Agriculture Organization of the United Nations, Rome (1988)Google Scholar