Extraction of polysaccharides under vacuum condition from Lentinus edodes stipe and their antioxidant activities in vitro

  • Shunfeng Li
  • Anjian Wang
  • Lina Liu
  • Guangrui Tian
  • Fangfang Xu


To improve the efficiency of polysaccharide extraction, vacuum technology was used to breakdown the cell wall to extract polysaccharides from Lentinus edodes stipe, and the optimal parameters were optimized by response surface methodology. Then the antioxidant activities in vitro of the polysaccharides were evaluated. Results showed that the optimal conditions for polysaccharide extraction were temperature of 62 °C, vacuum of 0.08 MPa, stirring speed of 1200 r/min, time of 25 min and material/liquid ratio of 1:26 (g/mL). Under these conditions, the yield of polysaccharide was 4.28%. The decolorization rate and deproteinization rate of polysaccharides treated by kaolin and hydrogen peroxide were higher than that of activated carbon. However, there was no significant influence on the polysaccharide retention rate, that was about 80%. Results also indicated that polysaccharide treated with activated carbon could obtain higher antioxidant activity.


Lentinus edodes stipe Polysaccharide Vacuum Extraction Antioxidant activity 



This work was financially supported by the Special Fund for Independent Innovation Research (Grant No. YUCAIKE [2018]9) established by Henan Academy of Agricultural Sciences and Scientific and Technological Project of Henan Province (No. 182102110276, 172102110085), China.

Supplementary material

10068_2018_513_MOESM1_ESM.docx (81 kb)
Supplementary material 1 (DOCX 81 kb)


  1. Andō A. On the components of pileus and stipe of dried shiitake (Lentinus edodes (Berk.) Sing): chemical components and hot water soluble polysaccharides. J. Home Econ. Jpn 27: 86–91 (1976)Google Scholar
  2. Bisen PS, Baghel RK, Sanodiya BS, Thakur GS, Prasad GBKS. Lentinus edodes: a macrofungus with pharmacological activities. Curr. Med. Chem. 17: 2419–2430 (2010)CrossRefGoogle Scholar
  3. Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72: 248–254 (1976)CrossRefGoogle Scholar
  4. Chen W, Li W, Yang Y, Yu H, Zhou S, Feng J, Li X, Liu Y. Analysis and evaluation of tasty components in the pileus and stipe of Lentinula edodes at different growth stages. J. Agric. Food Chem. 63: 795–801 (2015)CrossRefGoogle Scholar
  5. Chen H, Wang C, Ye J, Zhou H, Zhang Y, Tao R. Phenolic extracts from Rhus verniciflua stokes woods by vacuum extraction and their anti-tyrosinase activities. Chem. Ind. Forest Prod. 35: 47–54 (2015)Google Scholar
  6. China Edible Fungi Association. Notice on the results of 2016 annual output, output value and export statistics of edible fungus in China (China Edible Fungi Association [2017]11). Beijing (2017). Available from: Accessed 24 Oct 2017
  7. Darré M, Valerga L, Ortiz Araque LC, Lemoine ML, Demkura PV, Vicente AR, Concellón A. Role of UV-B irradiation dose and intensity on color retention and antioxidant elicitation in broccoli florets (Brassica oleracea var. Italica). Postharvest Biol. Tech. 128: 76–82 (2017)CrossRefGoogle Scholar
  8. Dermiki M, Phanphensophon N, Mottram DS, Methven L. Contributions of non-volatile and volatile compounds to the umami taste and overall flavour of shiitake mushroom extracts and their application as flavour enhancers in cooked minced meat. Food Chem. 141: 77–83 (2013)CrossRefGoogle Scholar
  9. Dubois M, Gilles K A, Hamilton JK, Rebers PA, Smith F. Colorimetric method for determination of sugars and related substances. Anal. Chem. 28: 350–356 (1956)CrossRefGoogle Scholar
  10. Gao H, Wang Y, Shi D, Wang S, He J, Guan J. Radio-frequency assisted extraction for polysaccharides from stems of shiitake mushroom. Trans. CSAE. 25: 180–184 (2009)Google Scholar
  11. Guo D, Ruan C, Liu H. Study on the extraction of water soluble polysaccharide from dried mushroom stem J. Heilongjiang Aug First Land Reclam Univ 13: 78–81 (2001)Google Scholar
  12. Huang Q, Yue T, Yuan Y, Wang Z. Optimization of ultrasonic-vacuum extraction of polysaccharides from fruit bodies of Pleurotus eryngii. Food Sci. 36: 77–82 (2015)Google Scholar
  13. Jiang Y. On the technology of ultrasonic-assisted polysaccharide extraction of shii-take stipe. Val. Eng. 3: 137–139 (2016)Google Scholar
  14. Li F, Chen L. Decoloring research on sweet potato leaf. J. Org. Chem. Res. 4: 82–92 (2016)CrossRefGoogle Scholar
  15. Li S, Wang A, Liu L, Tian G, Wei S, Xu F. Evaluation of nutritional values of shiitake mushroom (Lentinus edodes) stipes. J. Food Meas. Charact. 12: 2012–2019 (2018)CrossRefGoogle Scholar
  16. Lin L-Y, Tseng Y-H, Li R-C, Mau J-L. Quality of shiitake stipe bread. J. Food Process. Pres. 32: 1002–1015 (2008)CrossRefGoogle Scholar
  17. Lin P-H, Huang S-Y, Mau J-L, Liou B-K, Fang T J. A novel alcoholic beverage developed from shiitake stipe extract and cane sugar with various Saccharomyces strains. LWT Food Sci. Technol. 43: 971–976 (2010)CrossRefGoogle Scholar
  18. Ma C, Xia R, Peng Y, He X. A study of methods for extraction of lentinan and amino acids from the foot body of Lentinus edodes. Herald Med. 22: 372–374 (2003)Google Scholar
  19. Min F, Zhang Z, Qin Q, Ye J, Wang C. Optimization of vacuum cavitation extraction of tannic acid from Chinese gallnut using response surface method. Nat. Prod. Res. Dev. 25: 1240–1244 (2013)Google Scholar
  20. Qin N, Miao W-Y, Lian W-Q, Sun Y-L. Optimization of microwave-assisted extraction of polysaccharides from Lentinus edodes root by response surface analysis and its antioxidant activity. J. Food Saf. Qual. 8: 594–600 (2017)Google Scholar
  21. Rahman T, Choudhury M. Shiitake mushroom: A tool of medicine. Bangladesh J. Med. Biochem. 5: 24–32 (2012)Google Scholar
  22. Shi Q, Shao W. Determination of nutritive components of eight edible fungi. J. Gansu Agric. Univ. 38: 223–339 (2003)Google Scholar
  23. Shi D, Gao H, Zhou M, Cheng W, Wang S, Du X, Yang D. Effect of super fine crushing on the extraction yield of polysaccharide in Lentinus edodes stem. Hubei Agric. Sci. 48: 1730–1732 (2009)Google Scholar
  24. Tian L, Li H, Yuan Y, Yue T, Wei J. Optimization of ultrasound-vacuum assisted extraction of polyphenols from apple pomace. Food Sci. 38: 233–239 (2017)Google Scholar
  25. Tsai S-Y, Huang S-J, Mau J-L. Antioxidant properties of hot water extracts from Agrocybe cylindracea. Food Chem. 98: 670–677 (2006)CrossRefGoogle Scholar
  26. Wang J-X, Xiao X-H, Li G-K. Study of vacuum microwave-assisted extraction of polyphenolic compounds and pigment from Chinese herbs. J. Chromatogr. A 1198–1199: 45–53 (2008)CrossRefGoogle Scholar
  27. Wang Y, Xu L, Cao F, Wang W, Zhao F, Yang S, Lei M, Chen Y, Tang Y, Jiang G, Wang G. Negative pressure cavitation-assisted extraction and purification of proanthocyaidins from Ginkgo biloba leaves. Food Sci. 34: 6–10 (2013)Google Scholar
  28. Wang L, Cheng L, Xu Y, Wang T, Li L, Wang H. Extraction technology optimization and antioxidant activity of polysaccharides from pumpkin seeds. Trans. CSAE. 32(9): 284–290 (2016)Google Scholar
  29. Wang A, Li J, Wang X, Li S, Liu L, Tian G. Decolorization of dietary fiber from fermented Lentinus edodes stipe by the combination of ultrasound and H2O2. Packag. Food Mach. 35: 19–23 (2017)Google Scholar
  30. Xiao X-H, Wang J-X, Wang G, Wang J-Y, Li G-k. Evaluation of vacuum microwave-assisted extraction technique for the extraction of antioxidants from plant samples. J. Chromatogr. A 1216: 8867–8873 (2009)CrossRefGoogle Scholar
  31. Xu L, Xu G, Wang Z, Xiao W. Study on vacuum extraction process of honeysuckle. Chinese J. Exp. Tradit. Med. Formulae 17: 31–33 (2011)Google Scholar
  32. Yang J-H, Lin H-C, Mau J-L. Non-volatile taste components of several commercial mushrooms. Food Chem. 72: 465–471 (2001)CrossRefGoogle Scholar
  33. Yen M-T, Mau J-L. Preparation of fungal chitin and chitosan from shiitake stipes. Fungal Sci. 21: 1–11 (2006)Google Scholar
  34. Yen M-T, Mau J-L. Selected physical properties of chitin prepared from shiitake stipes. LWT Food Sci. Technol. 40: 558–563 (2007)CrossRefGoogle Scholar
  35. Yin C, Fan X, Fan Z, Shi D, Gao H. Optimization of enzymes-microwave-ultrasound assisted extraction of Lentinus edodes polysaccharides and determination of its antioxidant activity. Int. J. Biol. Macromol. 111: 446–454 (2018)CrossRefGoogle Scholar
  36. Zhang Z, Song H, Peng Z, Luo Q, Ming J, Zhao G. Characterization of stipe and cap powders of mushroom (Lentinus edodes) prepared by different grinding methods. J. Food Eng. 109: 406–413 (2012)CrossRefGoogle Scholar
  37. Zheng J, Ding X. Study on the antimutative PJF1 polysaccharide from foot body of Lentinus edodes. J. Wuxi Univ. Light Ind. 14: 7–13 (1995)Google Scholar
  38. Zhou H, Wang C, Ye J, Chen H, Tao R, Zhang Y. Optimization of decompressing ebullition extraction of total flavonoids from Ginkgo leaves. J. Northeast For. Univ. 43: 128–132 (2015)Google Scholar
  39. Zill-e-Huma, Abert-Vian M, Elmaataoui M, Chemat F. A novel idea in food extraction field: Study of vacuum microwave hydrodiffusion technique for by-products extraction. J. Food Eng. 105: 351–360 (2011)CrossRefGoogle Scholar
  40. Zu Y, Liu L, Xue Y, Shi Q. Extraction of astaxanthin by cacuum cavitation. J. Northeast For. Univ. 35: 59–60 (2007)Google Scholar

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© The Korean Society of Food Science and Technology and Springer Science+Business Media B.V., part of Springer Nature 2018

Authors and Affiliations

  1. 1.Institute of Agro-Products ProcessingHenan Academy of Agricultural SciencesZhengzhouPeople’s Republic of China

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