Food Science and Biotechnology

, Volume 28, Issue 1, pp 165–174 | Cite as

Enzymatic modification of daidzin using heterologously expressed amylosucrase in Bacillus subtilis

  • Eun-Ryoung Kim
  • Chan-Su Rha
  • Young Sung Jung
  • Jung-Min Choi
  • Gi-Tae Kim
  • Dong-Hyun Jung
  • Tae-Jip Kim
  • Dong-Ho Seo
  • Dae-Ok Kim
  • Cheon-Seok ParkEmail author


Amylosucrases (ASase, EC from Deinococcus geothermalis (DGAS) and Neisseria polysaccharea (NPAS) were heterologously expressed in Bacillus subtilis. While DGAS was successfully expressed, NPAS was not. Instead, NPAS was expressed in Escherichia coli. Recombinant DGAS and NPAS were purified using nickel-charged affinity chromatography and employed to modify daidzin to enhance its water solubility and bioavailability. Analyses by LC/MS revealed that the major products of transglycosylation using DGAS were daidzein diglucoside and daidzein triglucoside, whereas that obtained by NPAS was only daidzein diglucoside. The optimal bioconversion conditions for daidzein triglucoside, which was predicted to have the highest water-solubility among the daidzin derivatives, was determined to be 4% (w/v) sucrose and 250 mg/L daidzin in sodium phosphate pH 7.0, with a reaction time of 12 h. Taken together, we suggest that the yield and product specificity of isoflavone daidzin transglycosylation may be modulated by the source of ASase and reaction conditions.


Amylosucrase Deinococcus geothermalis Daidzin Transglycosylation Bacillus subtilis 



This work was supported by a National Research Foundation of Korea (NRF) Grant funded by the Korean Government (MEST) (No. 2017R1A2B4004218).

Compliance with ethical standards

Conflict of interest

The authors declare that there are no conflicts of interest.


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Copyright information

© The Korean Society of Food Science and Technology and Springer Science+Business Media B.V., part of Springer Nature 2018

Authors and Affiliations

  • Eun-Ryoung Kim
    • 1
  • Chan-Su Rha
    • 1
  • Young Sung Jung
    • 1
  • Jung-Min Choi
    • 1
  • Gi-Tae Kim
    • 1
  • Dong-Hyun Jung
    • 1
  • Tae-Jip Kim
    • 2
  • Dong-Ho Seo
    • 3
  • Dae-Ok Kim
    • 1
  • Cheon-Seok Park
    • 1
    Email author
  1. 1.Graduate School of Biotechnology and Institute of Life Science and ResourcesKyung Hee UniversityYonginRepublic of Korea
  2. 2.School of Food and Animal ScienceChungbuk National UniversityCheongjuRepublic of Korea
  3. 3.Research Group of HealthcareKorea Food Research InstituteWanjuRepublic of Korea

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