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Food Science and Biotechnology

, Volume 28, Issue 1, pp 175–180 | Cite as

Extracts from Erythronium japonicum and Corylopsis coreana Uyeki reduce 1,3-dichloro-2-propanol-mediated oxidative stress in human hepatic cells

  • Chun-Sik Bae
  • Chul-Ho Yun
  • Taeho AhnEmail author
Article
  • 83 Downloads

Abstract

In this study, it was demonstrated that 1,3-dichloro-2-propanol (1,3-DCP) induced oxidative stress and cell death in HuH7, human hepatocytes. The protective effects of Erythronium japonicum (E. japonicum) and Corylopsis coreana Uyeki (C. coreana Uyeki) extracts against 1,3-DCP-treated cells were also investigated. First, the activities of superoxide dismutase (SOD) and catalase (CAT) were diminished by the treatment of 1,3-DCP. Moreover, 1,3-DCP stimulated the expression and catalytic activity of cytochrome P450 2E1 (CYP2E1), an enzyme that generates reactive oxygen species in the liver. In contrast, co-treatment of 1,3-DCP with the extracts significantly decreased ROS generation and inhibited CYP2E1 activity without affecting its expression. The co-administration of extracts also restored the activities of SOD and CAT reduced by 1,3-DCP and protected against 1,3-DCP-mediated cell death. In conclusion, these results suggest that 1,3-DCP induces oxidative stress through the elevated CYP2E1 level, which is inhibited by the extracts, protecting cells against the effects of 1,3-DCP.

Keywords

Erythronium japonicum Corylopsis coreana Uyeki 1,3-Dichloro-2-propanol Cytochrome P450 2E1 HuH7 cells Oxidative stress 

Notes

Acknowledgements

This study was financially supported by Chonnam National University (Grant No. 2017-2787).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

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Copyright information

© The Korean Society of Food Science and Technology and Springer Science+Business Media B.V., part of Springer Nature 2018

Authors and Affiliations

  1. 1.College of Veterinary MedicineChonnam National UniversityGwangjuRepublic of Korea
  2. 2.School of Biological Sciences and TechnologyChonnam National UniversityGwangjuRepublic of Korea

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