Food Science and Biotechnology

, Volume 28, Issue 1, pp 111–120 | Cite as

Disruption of Phaffia rhodozyma cells and preparation of microencapsulated astaxanthin with high water solubility

  • Li Chen
  • Ji-Lian Wang
  • Hua Ni
  • Ming-Jun ZhuEmail author


A novel process was developed for encapsulation of astaxanthin from Phaffia rhodozyma. The yeast cells were disrupted by glass beads and the high shearing force partially emulsified the astaxanthin in aqueous phase. The enzymolysis method was then adopted to prepare the yeast extract for a full use of the cells. The gelatin and porous starch were used to microencapsulate the emulsified astaxanthin. Under optimized conditions, the recovery of amino nitrogen and solid reached 3.68 ± 0.32% and 49.22 ± 2.34%, respectively. The microencapsulation conditions were optimized through orthogonal experiment and the encapsulation efficiency, loading astaxanthin, and amino-nitrogen reached 88.56%, 1.55 mg/g, and 1.35 ± 0.14%, respectively. The water solubility of microcapsules reached 81.5 ± 0.35%. Color and storage stability analysis showed that microencapsulation of astaxanthin possessed higher thermal stability. The results demonstrated that the established process was effective and practical.


Disruption Bead milling Astaxanthin microencapsulation Porous starch Gelatin 



This article was financially supported by the State Key Laboratory of Pulp and Paper Engineering [Grant No. 2017TS06], National Natural Science Foundation of China [Grant No. 51478190], and Guangzhou Science and Technology Program [Grant No. 2014 Y2 -00515].

Compliance with ethical standards

Conflict of interest

The author declares that they have no conflict of interest.

Supplementary material

10068_2018_443_MOESM1_ESM.docx (316 kb)
Supplementary data (Fig S1 and Table S1-S2) associated with this article can be found in the online version (DOCX 315 kb)


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Copyright information

© The Korean Society of Food Science and Technology and Springer Science+Business Media B.V., part of Springer Nature 2018

Authors and Affiliations

  1. 1.Guangdong Engineering Center for Biopharmaceuticals, School of Biology and Biological EngineeringSouth China University of Technology, Guangzhou Higher Education Mega CenterPanyu, GuangzhouPeople’s Republic of China
  2. 2.State Key Laboratory of Pulp and Paper EngineeringSouth China University of TechnologyGuangzhouPeople’s Republic of China
  3. 3.College of Life and Geographic SciencesKashgar UniversityKashgarPeople’s Republic of China
  4. 4.The Key Laboratory of Ecology and Biological Resources in Yarkand Oasis at Colleges and Universities Under the Department of Education of Xinjiang Uygur Autonomous RegionKashgar UniversityKashgarPeople’s Republic of China

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