Food Science and Biotechnology

, Volume 28, Issue 1, pp 253–260 | Cite as

Aqueous extract of Lespedeza cuneata improves male menopause by increase of nitric oxide and dihydrotestosterone

  • Yun-Hee RheeEmail author
  • Sang-Woo Yoo
  • Seong Lee
  • Phil-Sang Chung


This study aimed to evaluate the effect of aqueous extract of Lespedeza cuneata (ALC) on men menopause induced erectile dysfunction (ED) and testosterone deficiency syndrome (TDS) in vitro and in vivo. Nitric oxide (NO) and cyclic guanosine monophosphate (cGMP) have been fundamental factors of ED, and free testosterone such as dihydrotestosterone (DHT) has a crucial role in mitigation of TDS. ALC increased the production of NO in a dose-dependent manner in vitro and in vivo. The level of neuronal nitric oxide synthase and cGMP was increased in the ALC group. The level of DHT and 17-beta hydroxysteroid dehydrogenases (17β-HSD) was also increased in the ALC group. There was no significant change of hepatotoxicity, renal toxicity, lipid metabolism, and glucose. These results suggest that ALC enhanced NO, cGMP and free testosterone which could be developed as a health supplement for improving male menopause.


Aqueous extract of Lespedeza cuneata (ALC) NO cGMP Free testosterone Male menopause 



This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (2017R1D1A1B03030060) and the Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry, and Fisheries (iPET) through the Technology Commercialization Support Program, funded by the Ministry of Agriculture, Food, and Rural Affairs (MAFRA) (816004-02-2), Republic of Korea.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no competing interests.


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Copyright information

© The Korean Society of Food Science and Technology and Springer Science+Business Media B.V., part of Springer Nature 2018

Authors and Affiliations

  1. 1.Beckman Laser Institute KoreaDankook UniversityCheonanRepublic of Korea
  2. 2.Natural Way Co. LtdPocheonRepublic of Korea
  3. 3.Laser Translational Clinical Trial CenterDankook University HospitalCheonanRepublic of Korea
  4. 4.Department of Otolaryngology-Head and Neck Surgery, College of MedicineDankook UniversityCheonanRepublic of Korea

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