Isolation of a sleep-promoting compound from Polygonatum sibiricum rhizome
- 22 Downloads
The aim of this study was to identify sleep-promoting substance from Polygonatum sibiricum rhizome extract (PSE) with the regulation of sleep architecture. PSE showed a decrease in sleep latency time and an increase in the sleeping time. In the electroencephalography analysis of rats, PSE (150 mg/kg) showed an increase of non-rapid eye movement by 38% and a decrease of rapid eye movement by 31% compared to the control. This sleep-promoting activity was found to be involved in the GABAA-BDZ receptor. The chemical structure of the pure compound was determined by the 1H and 13C nuclear magnetic resonance spectroscopy and gas chromatography mass spectrometry analysis; active compound was glyceryl-1-monolinoleate. The commercial standard glyceryl-1-monolinoleate showed a similar inhibitory concentration on [3H]-flumazenil binding to GABAA-BDZ receptors with final active fraction of PSE. The results indicate that glyceryl-1-monolinoleate is a major active compound responsible for the PSE-derived sleep promotion.
KeywordsGlyceryl monolinoleate Polygonatum sibiricum Insomnia Sleep GABA type A-benzodiazepine receptor
This research was supported by Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry (IPET) through the High Value-Added Food Technology Development Program, funded by Ministry of Agriculture, Food and Rural Affairs (MAFRA) (116004-02-2-HD020).
Compliance with ethical standards
Conflict of interest
The authors declare no conflict of interest.
- Attele AS, Xie JT, Yuan CS. Treatment of insomnia: an alternative approach. Altern. Med. Rev. 5: 249–259 (2000)Google Scholar
- Fang X, Hao JF, Zhou HY, Zhu LX, Wang JH, Song FQ. Pharmacological studies on the sedative-hypnotic effect of semen Ziziphi spinosae (Suanzaoren) and radix et rhizoma Salviae miltiorrhizae (Danshen) extracts and the synergistic effect of their combinations. Phytomedicine 17: 75–80 (2010)CrossRefGoogle Scholar
- Ghorbani A, Rakhshandeh H, Sadeghnia HR. Potentiating effects of Lactuca sativa on pentobarbital-induced sleep. Iran J. Pharm. Res. 12: 401–406 (2013)Google Scholar
- Heinrich M, Barnes J, Gibbons S, Williamson E. Fundamentals of pharmacognosy and phytotherapy. Churchill Livingstone, Elsevier Science Ltd., UK. (2004)Google Scholar
- Krueger JM, Kubillus S, Shai S, Davenne D. Enhancement of slow-wave sleep by endotoxin and lipid A. Am. J. Physiol. Regul. Integr. Comp. Physiol. 20: 7 (1986)Google Scholar
- McKenna JT, Zielinski MR, McCarley RW. Neurobiology of REM sleep, NREM sleep homeostasis, gamma band oscillations, Springer, New York, NY. pp 55–77 (2017)Google Scholar
- Srivastava JK, Shankar E, Gupta S. Chamomile: a herbal medicine of the past with bright future. Mol. Med. Report. 3: 895–901 (2010)Google Scholar
- Sun L, Li X. Studies on chemical constituents of Polygonatum sibiricum (II). Zhongcaoyao 32: 586–588 (2001)Google Scholar
- Yang JY, Wu CF, Song HR. Studies on the sedative and hypnotic effects of oleamide in mice. Arzneimittelforschung 49: 663–667 (1999)Google Scholar
- Yost CS, Hampson AJ, Leonoudakis D, Koblin DD, Bornheim LM, Gray AT. Oleamide potentiates benzodiazepine-sensitive gamma-aminobutyric acid receptor activity but does not alter minimum alveolar anesthetic concentration. Anesth. Analg. 86: 1294–1300 (1998)Google Scholar
- Zhang DY, Tashiro M, Shibuya K, Okamura N, Funaki Y, Yoshikawa T, Kato M, Yanai K. Next-day residual sedative effect after nighttime administration of an over-the-counter antihistamine sleep aid, diphenhydramine, measured by positron emission tomography. J. Clin. Psychopharmacol. 30: 694–701 (2010)CrossRefGoogle Scholar
- Zheng H, Dong Z, She J. Modernization of traditional chinese medicine and application. Xueyuan Press, Beijing, China. pp. 4071–4074 (1998)Google Scholar