Glycerides isolated from the aerial parts of Malva verticillata cause immunomodulation effects via splenocyte function and NK anti-tumor activity
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A preliminary study revealed that a 10 µg/mL n-BuOH fraction of Malva verticillata aerial parts significantly enhanced splenocyte proliferation and induced significant enhancement of natural-killer (NK) cell activity against tumor cells (YAC-1). This study was initiated to identify the principal components that exhibited these activities, and four glycerides were isolated through repeated SiO2 and ODS column chromatography. Structures of compounds 1–4 were determined to be (2S)-1-O-palmitoyl glyceride, (2S)-1-O-stearoyl glyceride, (2S)-1-O-linolenoyl glyceride, and (2S)-1,2-di-O-linoleoyl glyceride, respectively. Compounds 1–3 showed potential immune-enhancing activity in murine splenocyte and natural-killer (NK) cells at 10 µM. In contrast, compound 4 showed weak activity, indicating the monoacyl glycerides (1–3) are more effective than diacyl glyceride (4). Also, the longer the carbon number of the fatty acid in monoacyl glyceride, the better the activity, and the monoacyl glyceride including an unsaturated fatty acid (3) is more effective than the glycerides including the saturated fatty acids (1–2).
KeywordsMalva verticillata Glyceride Splenocyte Natural killer cells Immunotherapy
This work was supported by the Korea Evaluation Institute of Industrial Technology (Development of novel materials based on dual functions for improvement of atopic dermatitis and photoaging by regulating NFAT Grant number: 10076337), Republic of Korea.
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Conflict of interest
The authors declare no conflict of interest.
- 1.Jonas WB. Alternative medicine. J. Fam. Pract. 45:34–37 (1997)Google Scholar
- 4.Chu DT, Lepe-Zuniga J, Wong WL, LaPushin R, Mavligit GM. Fractionated extract of Astragalus membranaceus, a Chinese medicinal herb, potentiates LAK cell cytotoxicity generated by a low dose of recombinant interleukin-2. J Clin. Lab. Immunol. 26:183–187 (1988)Google Scholar
- 5.Chu DT, Lin JR, Wong W. The in vitro potentiation of LAK cell cytotoxicity in cancer and AIDS patients induced by F3-a fractionated extract of Astragalus membranaceus. Chung Hua Chung Liu Tsa Chih 16:167–171 (1994)Google Scholar
- 15.Abdel-Hamid NM, Fawzy MA, El-Moselhy MA. Evaluation of hepatoprotective and anticancer properties of aqueous olive leaf extract in chemically induced hepatocellular carcinoma in rats. Am. J. Med. Med. Sci. 1:15–22 (2011)Google Scholar
- 20.Hicks AM, Riedlinger G, Willingham MC, Alexander-Miller MA, Von Kap-Herr C, Pettenati MJ, Sanders AM, Weir MH, Du W, Kim J, Simpson AJG, Old LJ, Cui Z, Simpson AJ. Transferable anticancer innate immunity in spontaneous regression/complete resistance mice. Proc. Natl. Acad. Sci. 103:7753–7758 (2006)CrossRefGoogle Scholar
- 28.Kodali DR. Improved method for the synthesis of 1-or 3-acyl-sn-glycerols. J. Lipid Res. 28:464–469 (1987)Google Scholar