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Food Science and Biotechnology

, Volume 27, Issue 4, pp 1023–1030 | Cite as

Glycerides isolated from the aerial parts of Malva verticillata cause immunomodulation effects via splenocyte function and NK anti-tumor activity

  • Jung-Hwan Ko
  • Rodrigo Castaneda
  • Sun-Woo Joo
  • Hyoung-Geun Kim
  • Yeong-Geun Lee
  • Youn-Hyung Lee
  • Tong Ho Kang
  • Nam-In Baek
Article

Abstract

A preliminary study revealed that a 10 µg/mL n-BuOH fraction of Malva verticillata aerial parts significantly enhanced splenocyte proliferation and induced significant enhancement of natural-killer (NK) cell activity against tumor cells (YAC-1). This study was initiated to identify the principal components that exhibited these activities, and four glycerides were isolated through repeated SiO2 and ODS column chromatography. Structures of compounds 14 were determined to be (2S)-1-O-palmitoyl glyceride, (2S)-1-O-stearoyl glyceride, (2S)-1-O-linolenoyl glyceride, and (2S)-1,2-di-O-linoleoyl glyceride, respectively. Compounds 13 showed potential immune-enhancing activity in murine splenocyte and natural-killer (NK) cells at 10 µM. In contrast, compound 4 showed weak activity, indicating the monoacyl glycerides (13) are more effective than diacyl glyceride (4). Also, the longer the carbon number of the fatty acid in monoacyl glyceride, the better the activity, and the monoacyl glyceride including an unsaturated fatty acid (3) is more effective than the glycerides including the saturated fatty acids (12).

Keywords

Malva verticillata Glyceride Splenocyte Natural killer cells Immunotherapy 

Notes

Acknowledgements

This work was supported by the Korea Evaluation Institute of Industrial Technology (Development of novel materials based on dual functions for improvement of atopic dermatitis and photoaging by regulating NFAT Grant number: 10076337), Republic of Korea.

Compliance with ethical standards

Conflict of interest

The authors declare no conflict of interest.

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Copyright information

© The Korean Society of Food Science and Technology and Springer Science+Business Media B.V., part of Springer Nature 2018

Authors and Affiliations

  • Jung-Hwan Ko
    • 1
  • Rodrigo Castaneda
    • 1
  • Sun-Woo Joo
    • 1
  • Hyoung-Geun Kim
    • 1
  • Yeong-Geun Lee
    • 1
  • Youn-Hyung Lee
    • 2
  • Tong Ho Kang
    • 1
  • Nam-In Baek
    • 1
  1. 1.Department of Oriental Medicine Biotechnology, Graduate School of BiotechnologyKyung-Hee UniversityYonginRepublic of Korea
  2. 2.Department of Horticultural Biotechnology, Graduate School of BiotechnologyKyung-Hee UniversityYonginRepublic of Korea

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