Food Science and Biotechnology

, Volume 27, Issue 2, pp 461–466 | Cite as

New route of chitosan extraction from blue crabs and shrimp shells as flocculants on soybean solutes

  • Yongjae LeeEmail author
  • Hyun-Wook Kim
  • Yuan H. Brad Kim


The chitosan extracted from blue crabs and shrimp shells using calcium oxide (deproteinization) followed by deacetylation which eliminated the demineralization step to reduce the chemical usage and environmental protection. The extracted chitosan examined the flocculation to soybean solutes. The optical density (OD), solid%, and purity% (carbohydrates/soluble solids) after flocculation were measured. The OD was significantly decreased from 0.76 to 0.16 with blue crabs and 0.06 with shrimp shells chitosan-acetate dosing (0.5 g/L). The removal of about 68 and 66% solids was achieved by the addition of 0.5 g/L chitosan-acetate. The purity% was reached about 80% with blue crabs, and 78% with shrimp shells chitosan-acetate. The results of this study verified that the calcium oxide treatment should remove protein and increase the chitin extraction yield on blue crab and shrimp shells. This new route of chitosan extraction should be a useful method for making flocculants in the soybean solutes.


Chitosan Flocculants Calcium oxide Blue crabs Shrimp shell 



The authors wish to thank Process Engineering R&D Center staff members at Texas A&M University for equipment and technical support for this work.


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Copyright information

© The Korean Society of Food Science and Technology and Springer Science+Business Media B.V., part of Springer Nature 2017

Authors and Affiliations

  1. 1.Department of Nutrition and Food ScienceTexas A&M UniversityCollege StationUSA
  2. 2.Department of Animal Science and BiotechnologyGyeongnam National University of Science and TechnologyJinjuSouth Korea
  3. 3.Meat Science and Muscle Biology Laboratory, Department of Animal SciencesPurdue UniversityWest LafayetteUSA

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