Food Science and Biotechnology

, Volume 27, Issue 2, pp 525–535 | Cite as

Development of a novel fermented pumpkin-based beverage inoculated with water kefir grains: a response surface methodology approach

  • Wee Yin Koh
  • Uthumporn Utra
  • Ahmad Rosma
  • Mohd. Esah Effarizah
  • Wan Ishak Wan Rosli
  • Yong-Ha Park
Article
  • 67 Downloads

Abstract

Pumpkin (Cucurbita pepo) is well known for its health and nutritional benefits and is recommended for daily consumption. This is the first report on optimization and development of fermented pumpkin-based water kefir beverage. Optimum pumpkin puree and brown sugar concentrations were found at 22.28 and 9.07% w/v, respectively, were made into a pumpkin-based beverage and fermented with water kefir grains for 24 h at 32 °C. The optimized fermented pumpkin-based water kefir beverage was found to be non-alcoholic, achieved good overall acceptability and high Lactobacillus, acetic acid bacteria and yeast cell viability of approximately 1012, 109 and 109 CFU mL−1, respectively. Overall, the optimized product attained superb technological characteristics and has the potential for industrial exploitation as a refreshing water kefir drink.

Keywords

Box–Behnken design Cucurbita pepo Lactobacillus Water kefir Yeast 

Notes

Acknowledgements

The authors are grateful to the ASEAN University Network (AUN) and Korea Association of the Southeast Asian Studies for providing financial support under ASEAN-ROK Academic Exchange Programme 2016/2017; Research Universiti Grant (1001/PTEKIND/811339) and Universiti Sains Malaysia Vice-Chancellor Award Scholarship.

References

  1. 1.
    Li X-J, Li Z-G, Wang X, Han J-Y, Zhang B, Fu Y-J, Zhao C-J. Application of cavitation system to accelerate aqueous enzymatic extraction of seed oil from Cucurbita pepo L. and evaluation of hypoglycemic effect. Food Chem. 212: 403–410 (2016)CrossRefGoogle Scholar
  2. 2.
    Sedigheh A, Jamal MS, Mahbubeh S, Somayeh K, Mahmoud RK, Azadeh A, Fatemeh S. Hypoglycaemic and hypolipidemic effects of pumpkin (Cucurbita pepo L.) on alloxan-induced diabetic rats. African J. Pharmacy Pharmacol. 5: 2620–2626 (2011)Google Scholar
  3. 3.
    Ratz-Łyko A, Arct J. Evaluation of antioxidant and antimicrobial properties of enzymatically hydrolysed Cucurbita pepo and Linum usitatissimum seedcakes. Food Sci Biotechnol. 24: 1789–1796 (2015)CrossRefGoogle Scholar
  4. 4.
    Gutierrez RMP. Review of Cucurbita pepo (pumpkin) its phytochemistry and pharmacology. Med. Chem. 6: 12–21 (2016)CrossRefGoogle Scholar
  5. 5.
    Muzzaffar S, Baba WN, Nazir N, Masoodi FA, Bhat MM, Bazaz R. Effect of storage on physicochemical, microbial and antioxidant properties of pumpkin (Cucurbita moschata) candy. Cogent Food Agri. 2: 1163650 (2016)Google Scholar
  6. 6.
    Terefe, NS. Food Fermentation. Reference Module in Food Science. Elsevier (2016)Google Scholar
  7. 7.
    Zhao J, Liu W, Chen D, Zhou C, Song Y, Zhang Y,. Ni Q, Li Q. Microbiological and physicochemical analysis of pumpkin juice fermentation by the basidiomycetous fungus Ganoderma lucidum. J. Food Sci. 80: 241–251 (2015).CrossRefGoogle Scholar
  8. 8.
    Genevois C, Flores S, de Escalada Pla M. Byproduct from pumpkin (Cucurbita moschata Duchesne ex poiret) as a substrate and vegetable matrix to contain Lactobacillus casei. J. Func. Foods. 23: 210–219 (2016)CrossRefGoogle Scholar
  9. 9.
    Puerari C, Magalhães KT, Schwan RF. New cocoa pulp-based kefir beverages: Microbiological, chemical composition and sensory analysis. Food Res. Int. 48: 634–640 (2012)CrossRefGoogle Scholar
  10. 10.
    Gulitz A, Stadie J, Ehrmann MA, Ludwig W, Vogel RF. Comparative phylobiomic analysis of the bacterial community of water kefir by 16S rRNA gene amplicon sequencing and ARDRA analysis. J. Appl. Microbiol. 114: 1082–1091 (2013)CrossRefGoogle Scholar
  11. 11.
    Wang L, Zhong H, Liu K, Guo A, Qi X, Cai M, The evaluation of kefir pure culture starter: Liquid-core capsule entrapping microorganisms isolated from kefir grains. Food Sci. Technol. Int. 0: 1–11 (2016)Google Scholar
  12. 12.
    Corona O, Randazzo W, Miceli A, Guarcello R, Francesca N, Erten H, Moschetti G, Settanni L. Characterization of kefir-like beverages produced from vegetable juices. LWT-Food Sci. Tech. 66: 572–581 (2016)CrossRefGoogle Scholar
  13. 13.
    Sabokbar N, Khodaiyan F, Moosavi-Nasab M. Optimization of processing conditions to improve antioxidant activities of apple juice and whey based novel beverage fermented by kefir grains. J. Food Sci. Tech. 52: 3422–3432 (2015)Google Scholar
  14. 14.
    FAO/WHO. 2011. Milk and Milk Products. CODEX Standard for Fermented Milks (CODEX STAN 243-2003). Available from http://www.fao.org/docrep/015/i2085e/i2085e00.pdf. Accessed Sept. 05, 2017.
  15. 15.
    Balabanova T, Panayotov P. Obtaining functional fermented beverages by using the kefir grains. Procedia Food Sci. 1: 1653–1659 (2011)CrossRefGoogle Scholar
  16. 16.
    Grønnevik H, Falstad M, Narvhus JA. Microbiological and chemical properties of Norwegian kefir during storage. Int. Dairy J. 21: 601–606 (2011)CrossRefGoogle Scholar
  17. 17.
    Satir G, Guzel-Seydim ZB. Influence of Kefir fermentation on the bioactive substances of different breed goat milks. LWT - Food Sci. Technol. 63: 852–858 (2015)CrossRefGoogle Scholar
  18. 18.
    Fiorda FA, Vinicius de Melo Pereira G, Thomaz-Soccol V, Rakshit SK, Binder Pagnoncelli MG, Porto de Souza Vandenberghe L, Soccol CR, Vinicius G, Pereira DM, Assumpç F, Thomaz-Soccol V, Kumar S, Giovana M, Pagnoncelli B, Porto L, Vandenberghe DS, Ricardo C. Microbiological, biochemical, and functional aspects of sugary kefir fermentation - A review. Food Microbiol. 66: 86–95 (2017)CrossRefGoogle Scholar
  19. 19.
    Singh O, Panesar PS, Chopra HK. Response surface optimization for cellulose production from agro industrial waste by using new bacterial isolate Gluconacetobacter xylinus C18. Food Sci Biotechnol. 26:1019–1028 (2017)CrossRefGoogle Scholar
  20. 20.
    Sabokbar N, Moosavi-Nasab M, Khodaiyan F. Preparation and characterization of an apple juice and whey based novel beverage fermented using kefir grains. Food Sci. Biotechnol. 24: 2095–2104 (2015)CrossRefGoogle Scholar
  21. 21.
    Braga CM, Zielinski AAF, Da Silva KM, De Souza FKF, De Arruda Moura Pietrowski G, Couto M, Granato D, Wosiacki G, Nogueira A. Classification of juices and fermented beverages made from unripe, ripe and senescent apples based on the aromatic profile using chemometrics. Food Chem. 141: 967–974 (2013)CrossRefGoogle Scholar
  22. 22.
    Kazemalilou S, Alizadeh A. Optimization of Sugar Replacement with Date Syrup in Prebiotic Chocolate Milk Using Response Surface Methodology. Korea J. Food Sci. Anim. Resour. 37: 449–455 (2017)CrossRefGoogle Scholar
  23. 23.
    AOAC. Official Methods of Analysis, 21st edn. Association of official analytical chemists, Washington, DC, USA (1999).Google Scholar
  24. 24.
    Batista ÂG, da Silva JK, Cazarin CBB, Biasoto ACT, Sawaya ACHF, Prado MA, Júnior MRM. Red-jambo (Syzygium malaccense): Bioactive compounds in fruits and leaves. LWT-Food Sci. Tech. 76: 284–291 (2017)CrossRefGoogle Scholar
  25. 25.
    Du B, Song Y, Hu X, Liao X, Ni Y, Li Q. Oligosaccharides prepared by acid hydrolysis of polysaccharides from pumpkin (Cucurbita moschata) pulp and their prebiotic activities. Int. J. Food Sci. Tech. 46: 982–987 (2011)CrossRefGoogle Scholar
  26. 26.
    John SM, Deeseenthum S. Properties and benefits of kefir -A review. Songklanakarin J. Sci. Technol. 37: 275–282 (2015)Google Scholar
  27. 27.
    Mishra Pandey S, Mishra HN. Optimization of the prebiotic & probiotic concentration and incubation temperature for the preparation of synbiotic soy yoghurt using response surface methodology. LWT - Food Sci. Technol. 62: 458–467 (2015)CrossRefGoogle Scholar
  28. 28.
    Garner MR, Flint J. Process and composition for the manufacture of a microbial-based product. U.S. Patent 8,445,226 (2013)Google Scholar
  29. 29.
    Najiha AA, Tajul AY, Norziah MH, Wan Nadiah WA. A preliminary study on halal limits for ethanol content in food products. Middle-East J. Scientific Res. 6: 45–50 (2010)Google Scholar
  30. 30.
    Atalar I, Dervisoglu M. Optimization of spray drying process parameters for kefir powder using response surface methodology. LWT - Food Sci. Technol. 60: 751–757 (2015)CrossRefGoogle Scholar
  31. 31.
    Gulitz A, Stadie J, Wenning M, Ehrmann MA, Vogel RF. The microbial diversity of water kefir. Int. J. Food Microbiol. 151: 284–288 (2011)CrossRefGoogle Scholar
  32. 32.
    Miguel MGCP, Cardoso PG, Magalhães-Guedes KT, Schwan RF. Identification and assessment of kefir yeast potential for sugar/ethanol-resistance. Brazilian J. Microbiol. 44: 113–118 (2013)CrossRefGoogle Scholar
  33. 33.
    Yüksekdag ZN, Beyatli Y, Aslim B. Determination of some characteristics coccoid forms of lactic acid bacteria isolated from Turkish kefirs with natural probiotic. LWT - Food Sci. Technol. 37: 663–667 (2004)CrossRefGoogle Scholar
  34. 34.
    Muganga L, Liu X, Tian F, Zhao J, Zhang H, Chen W. Screening for lactic acid bacteria based on antihyperglycaemic and probiotic potential and application in synbiotic set yoghurt. J. Funct. Foods. 16: 125–136 (2015)CrossRefGoogle Scholar
  35. 35.
    Furukawa S, Watanabe T, Toyama H, Morinaga Y. Significance of microbial symbiotic coexistence in traditional fermentation. J. Biosci. Bioeng. 116: 533–539 (2013)CrossRefGoogle Scholar

Copyright information

© The Korean Society of Food Science and Technology and Springer Science+Business Media B.V. 2017

Authors and Affiliations

  • Wee Yin Koh
    • 1
  • Uthumporn Utra
    • 1
  • Ahmad Rosma
    • 2
  • Mohd. Esah Effarizah
    • 1
  • Wan Ishak Wan Rosli
    • 3
  • Yong-Ha Park
    • 4
  1. 1.Food Technology Division, School of Industrial TechnologyUniversiti Sains MalaysiaMindenMalaysia
  2. 2.Bioprocess Technology Division, School of Industrial TechnologyUniversiti Sains MalaysiaMindenMalaysia
  3. 3.School of Health Sciences, Health Sciences CampusUniversiti Sains MalaysiaKubang KerianMalaysia
  4. 4.Department of Applied Microbiology and BiotechnologyYeungnam UniversityGyeongsanSouth Korea

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