Food Science and Biotechnology

, Volume 26, Issue 2, pp 435–440 | Cite as

Effects of ozone treatment on physicochemical properties of Korean wheat flour

  • Min Jung Lee
  • Mi Jeong Kim
  • Han Sub Kwak
  • Seung-Taik Lim
  • Sang Sook Kim


The objective of this study was to investigate the effects of ozone treatment on the physicochemical properties of Korean wheat flour. Wheat flour samples were treated with ozone gas at 120 ppm for 15, 30, 45, and 60 min. Color b value, pH, and mold of flour decreased as exposure time to ozone increased. The water absorption index, peak viscosity, and final viscosity of flour increased by ozone treatment. Photomicrographs of flour suspensions under polarized light showed granules tended to lose birefringence owing to ozone during swelling. The result of SDS-PAGE showed that the intensity of protein bands at low molecular weights slightly increased in ozone-treated flours compared to the intensity in the control flour. The results of this study showed ozone gas affected the starch and protein of wheat flour, suggesting a need for further investigation on structural changes in starch and protein by ozone.


wheat ozone flour starch protein 


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  1. 1.
    He M, Zhu C, Dong K, Zhang T, Cheng Z, Li J, Yan Y. Comparative proteome analysis of embryo and endosperm reveals central differential expression proteins involved in wheat seed germination. BMC Plant Biol. 15: 1–17 (2015)CrossRefGoogle Scholar
  2. 2.
    FAO (Food and Agrigulture Organization of the United Nations), Food outlook. Available from: Accessed Sep. 20, 2016.Google Scholar
  3. 3.
    Park SH, Wilson JD, Seabourn BW. Starch granule size distribution of hard red winter and hard red spring wheat: Its effects on mixing and breadmaking quality. J. Cereal Sci. 49: 98–105 (2009)CrossRefGoogle Scholar
  4. 4.
    Delcour JA, Hoseney RC. Principles of cereal science and technology. American Association of Cereal Chemists, St. Paul, MN, USA. p. 53 (2010)CrossRefGoogle Scholar
  5. 5.
    Maningat C, Seib P, Bassi S, Woo K, Lasater G. Wheat starch: Production, properties, modification and uses. Starch: Chemistry and technology, 3rd ed. Elsevier Inc., New York City, NY, USA. pp. 441–510 (2009)Google Scholar
  6. 6.
    Kim MJ, Kim SS. Determination of the optimum mixture of transglutaminase, L-ascorbic acid and xylanase for the quality and consumer acceptability of bread using response surface methodology. Food Sci. Biotechnol. 25: 77–84 (2016)CrossRefGoogle Scholar
  7. 7.
    Curtis BC, Rajaram S, Gomez Macpherson H. Bread wheat: Improvement and production. pp. 6–16. FAO (Food and Agriculture Organization of the United Nations), Rome, Italy (2002)Google Scholar
  8. 8.
    Tanaka A, Takahashi K, Masutomi Y, Hanasaki N, Hijioka Y, Shiogama H, Yamanaka Y. Adaptation pathways of global wheat production: Importance of strategic adaptation to climate change. Sci. Rep. 5: Article number: 14312 (2015)CrossRefGoogle Scholar
  9. 9.
    Sandhu HPS, Manthey FA, Simsek S. Quality of bread made from ozonated wheat (Triticum aestivum L.) flour. J. Sci. Food Agr. 91: 1576–1584 (2011)CrossRefGoogle Scholar
  10. 10.
    Sandhu HPS, Manthey FA, Simsek S, Ohm JB. Comparison between potassium bromate and ozone as flour oxidants in breadmaking. Cereal Chem. 88: 103–108 (2011)CrossRefGoogle Scholar
  11. 11.
    Jurado-Alameda E, Garcia-Roman M, Altmajer-Vaz D, Jimenez-Perez JL. Assessment of the use of ozone for cleaning fatty soils in the food industry. J. Food Eng. 110: 44–52 (2012)CrossRefGoogle Scholar
  12. 12.
    Guzel-Seydim ZB, Greene AK, Seydim AC. Use of ozone in the food industry. Lebensm.-Wiss. Technol. 37: 453–460 (2004)CrossRefGoogle Scholar
  13. 13.
    Patil S, Torres B, Tiwari BK, Wijngaard HH, Bourke P, Cullen PJ, O’Donnell CP, Valdramidis VP. Safety and quality assessment during the ozonation of cloudy apple juice. J. Food Sci. 75: M437–M443 (2010)CrossRefGoogle Scholar
  14. 14.
    Li M, Zhu KX, Wang BW, Guo XN, Peng W, Zhou HM. Evaluation the quality characteristics of wheat flour and shelf-life of fresh noodles as affected by ozone treatment. Food Chem. 135: 2163–2169 (2012)CrossRefGoogle Scholar
  15. 15.
    Dhillon B, Wiesenborn D, Dhillon H, Wolf-Hall C. Development and evaluation of a fluidized bed system for wheat grain disinfection. J. Food Sci. 75: E372–E378 (2010)CrossRefGoogle Scholar
  16. 16.
    AACC. Approved Method of the AACC. 10th ed. Methods 02-52, 46-12, 38-12A, and 76-21. American Association of Cereal Chemists, St. Paul, MN, USA (2000)Google Scholar
  17. 17.
    AACC. International Approved Method of analysis. 11th ed. Method 76-33. American Association of Cereal Chemists, St. Paul, MN, USA (2010)Google Scholar
  18. 18.
    Anderson RA, Conway HF, Peplinsk Aj. Gelatinization of corn grits by roll cooking, extrusion cooking and steaming. Starke 22: 130–135 (1970)CrossRefGoogle Scholar
  19. 19.
    Ibanoglu S. Influence of tempering with ozonated water on the selected properties of wheat flour. J. Food Eng. 48: 345–350 (2001)CrossRefGoogle Scholar
  20. 20.
    Chittrakorn S, Earls D, MacRitchie F. Ozonation as an alternative to chlorination for soft wheat flours. J. Cereal Sci. 60: 217–221 (2014)CrossRefGoogle Scholar
  21. 21.
    Langlais B, Reckhow DA, Brink DR. Ozone in water treatment application and engineering. p. 62, CRP press, Inc., Boca Raton, FL, USA (1991)Google Scholar
  22. 22.
    Li M, Peng J, Zhu KX, Guo XN, Zhang M, Peng W, Zhou HM. Delineating the microbial and physical-chemical changes during storage of ozone treated wheat flour. Innov. Food Sci. Emerg. 20: 223–229 (2013)CrossRefGoogle Scholar
  23. 23.
    Eglezos S. Microbiological quality of wheat grain and flour from two mills in Queensland, Australia. J. Food Protect. 73: 1533–1536 (2010)CrossRefGoogle Scholar
  24. 24.
    Zorlugenc B, Zorlugenc FK, Oztekin S, Evliya IB. The influence of gaseous ozone and ozonated water on microbial flora and degradation of aflatoxin B(1) in dried figs. Food Chem. Toxicol. 46: 3593–3597 (2008)CrossRefGoogle Scholar
  25. 25.
    Pleijel H, Mortensen L, Fuhrer J, Ojanpera K, Danielsson H. Grain protein accumulation in relation to grain yield of spring wheat (Triticum aestivum L.) grown in open-top chambers with different concentrations of ozone, carbon dioxide and water availability. Agr. Ecosyst. Environ. 72: 265–270 (1999)CrossRefGoogle Scholar
  26. 26.
    Zhou XD, Zhou J, Wang YX, Peng B, Zhu JG, Yang LX, Wang YL. Elevated tropospheric ozone increased grain protein and amino acid content of a hybrid rice without manipulation by planting density. J. Sci. Food Agr. 95: 72–78 (2015)CrossRefGoogle Scholar
  27. 27.
    Mei J, Liu G, Huang X, Ding W. Effects of ozone treatment on medium hard wheat (Triticum aestivum L.) flour quality and performance in steamed bread making. CyTA J. Food 14: 449–456 (2016)Google Scholar
  28. 28.
    Chan HT, Bhat R, Karim AA. Physicochemical and functional properties of ozone-oxidized starch. J. Agr. Food Chem. 57: 5965–5970 (2009)CrossRefGoogle Scholar
  29. 29.
    Sebecic B, Sebecic B. Wheat-flour starch granule-size distribution and rheological properties of dough. 1. Granulometric analysis of starch. Nahrung 39: 106–116 (1995)CrossRefGoogle Scholar
  30. 30.
    Catal H, Ibanoglu S. Ozonation of corn and potato starch in aqueous solution: Effects on the thermal, pasting and structural properties. Int. J. Food Sci. Tech. 47: 1958–1963 (2012)CrossRefGoogle Scholar
  31. 31.
    Kamal AHM, Kim KH, Shin KH, Seo HS, Tsujimoto H, Heo HY, Choi JS, Park CS, Woo SH. Diversity of novel glutenin subunits in bread wheat (Triticum aestivum L.). J. Plant Biol. 52: 533–542 (2009)CrossRefGoogle Scholar

Copyright information

© The Korean Society of Food Science and Technology and Springer Science+Business Media Dordrecht 2017

Authors and Affiliations

  • Min Jung Lee
    • 1
    • 2
  • Mi Jeong Kim
    • 1
  • Han Sub Kwak
    • 1
  • Seung-Taik Lim
    • 2
  • Sang Sook Kim
    • 1
  1. 1.Research Group of Cognition and Sensory PerceptionKorea Food Research InstituteSeongnam, GyeonggiKorea
  2. 2.Department of Biotechnology, College of Life Sciences and BiotechnologyKorea UniversitySeoulKorea

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